2026-03-10T21:46:26Z https://fra.repo.nii.ac.jp/oai

oai:fra.repo.nii.ac.jp:02007610 2026-02-03T06:54:23Z 12:14:1716955633551

アマゴ血清中の凝集素価に及ぼす非免疫原菌体吸収操作の影響 Effect of Absorption by Non-Specific Bacterial Cells on Specific Agglutinin Activity in Serum of Amago Salmon (Oncorhynchus rhodurus) 30371842 Ikeda, Kazuo 池田, 和夫 イケダ, カズオ Ikeda 池田 イケダ Kazuo 和夫 カズオ metadata only access absorption; Aeromonas hydrophila; Aeromonas salmonicida; agglutinin; hemolysis Effect of preliminary absorption by Aeromonas hydrophila on agglutinin activity to Aeromonas salmonicida in serum of amago salmon (Oncorhynchus rhodurus) was studied. Amago salmon were immunized by an intraperitoneal injection of formalinized A. salmonicida strain Gifu. After 36 days, the blood was taken from immunized and control amago salmon. For all sera, a portion of the serum was absorbed by A. hydrophila strain CB-1, and the agglutinin activity to A. salmonicida and hemolysis activity to sheep red blood cells (SRBC) were measured for both absorbed and non-treated sera. Agglutinin activity to A. salmonicida in the A. salmonicida immunized serum was not affected by the preliminary absorption with A. hydrophila, but hemolysis activity to SRBC disappeared by the absorption. Both agglutinin-like activity to A. salmonicida and hemolysis activity of control serum were moderately high before absorption, but these activities almost disappeared after the absorption. These results suggest that there was the non-specific bacteriolytic and hemolytic substance(s) which was absorbed or consumed by mixing the serum with A. hydrophila. Since the non-specific bacteriolytic and hemolytic substance(s) may cause higher agglutinin and hemolytic titers, preliminary absorption, such as absorption by non-specific bacterial cells is necessary for the specific determination of agglutinin activity of amago salmon sera, especially for the sera of low level titers. 養殖研究所 1990-03-31 jpn departmental bulletin paper https://fra.repo.nii.ac.jp/records/2007610 nria_k_17_51 https://agriknowledge.affrc.go.jp/RN/2010461295 日本農学文献記事索引(agriknowledge) AN00245945 0389-5858 養殖研究所研究報告 Bulletin of National Research Institute of Aquaculture 17 4 51 54