2026-03-07T05:49:56Z https://fra.repo.nii.ac.jp/oai

oai:fra.repo.nii.ac.jp:02008146 2025-12-10T06:43:16Z 12:14:1716951205967

ナラワスサビノリ糸状体の超低温保存における前培養条件の検討 Preculturing Conditions for Cryopreservation of Conchocelis of Porphyra yezoensis form. narawaensis 80372001 Fujiyoshi, Eiji 藤吉, 栄次 フジヨシ, エイジ Fujiyoshi 藤吉 フジヨシ Eiji 栄次 エイジ Umezawa, Satoshi 梅澤, 敏 ウメザワ, サトシ Umezawa 梅澤 ウメザワ Satoshi 敏 サトシ metadata only access Various preculturing conditions were studied in order to improve the survival rates of conchocelis cells of Porphyra yezoensis form. narawaensis after freeze-thaw cycle. The preculturing conditions tested were combinations of temperature (6℃ or 18℃), period (1 or 3 days) and with or without cryoprotectant (0.5 M sorbitol, 0.9 M sorbitol or 0.5M DMSO). The precultured cells were then treated with the cryoprotective solution containing 1.5M DMSO and 0.5 M sorbitol, cooled at 1℃/ min to -45℃ and plunged into liquid nitrogen. After the freeze-thaw cycle, the survival rates of the precultured cells were determined. It was found that preculturing cells with 0.5 M sorbitol, 0.9 M sorbitol or 0.5 M DMSO at 6℃ for 3 days and with 0.5 M sorbitol at 18°C for 1 day improved the survival rate as much as 20% compared with the cells precultured without cryoprotectant at 18℃. 西海区水産研究所 Seikai National Fisheries Research Institute 1997-12 jpn departmental bulletin paper https://fra.repo.nii.ac.jp/records/2008146 snf_k_75_55 https://agriknowledge.affrc.go.jp/RN/2010570985 日本農学文献記事索引(agriknowledge) AN00393385 0582-415X 西海区水産研究所研究報告 Bulletin of the Seikai National Fisheries Research Institute 75 5 55 59