2026-03-07T05:36:52Z https://fra.repo.nii.ac.jp/oai

oai:fra.repo.nii.ac.jp:02012153 2026-02-19T01:43:36Z 1:6:1724312737826

マダイ筋原線維結合型セリンプロテアーゼの同定 Identification of Myofibril‒bound Serine Protease from Red Seabream（Pagrus major）Ordinary Muscle 60381092 Okubo, Makoto 大久保, 誠 オオクボ, マコト Okubo 大久保 オオクボ Makoto 誠 マコト 10549942 Yaguchi, Shigenori 谷口, 成紀 ヤグチ, シゲノリ Yaguchi 谷口 ヤグチ Shigenori 成紀 シゲノリ 70399666 Kondo, Masakazu 近藤, 昌和 コンドウ, マサカズ Kondo 近藤 コンドウ Masakazu 昌和 マサカズ 20399653 Maeda, Toshimichi 前田, 俊道 マエダ, トシミチ Maeda 前田 マエダ Toshimichi 俊道 トシミチ open access red seabream; serine protease; modori; kamaboko Myofibrillar proteins were prepared from red seabream (Pagrus major) ordinary muscle by washing four times to remove sarcoplasmic proteins. Myosin heavy chain (MHC) of the myofibrillar proteins was degraded at 55℃ without the addition of any protease, and the degree of MHC degradation in red seabream was obviously low in comparison with brushtooth lizardfish (Saurida sp.). Specific activity of the red seabream myofibrillar proteins was approximately one-thirtieth that of brushtooth lizardfish. Red seabream myofibril-bound serine protease (MBSP) was solubilized by heat treatment of the washed myofibrillar proteins at 55℃ for 10 min. Hydrolysis of synthetic fluorogenic substrate by both the myofibril-bound and solubilized MBSPs was inhibited by Pefabloc SC, a serine protease inhibitor. Both MBSPs rapidly hydrolyzed synthetic fluorogenic substrates containing arginine residues at the P1 position. These results indicate that red seabream MBSP is a trypsin-type serine protease, as reported in other fish species, such as white croaker (Pennahia argentata) and brushtooth lizardfish, utilized as ingredients of kamaboko. 編集：水産大学校 水産研究・教育機構 Japan Fisheries Research and Education Agency 2019-11-22 eng departmental bulletin paper https://fra.repo.nii.ac.jp/records/2012153 fishu_k_68_1 https://agriknowledge.affrc.go.jp/RN/2010932828 agriknowledge AN00124678 0370-9361 水産大学校研究報告 Journal of National Fisheries University 68 1 9 1 9 https://fra.repo.nii.ac.jp/record/2012153/files/fishu_k_68_1.pdf application/pdf 656.1 KB 2024-10-11