2026-03-05T16:39:29Z https://fra.repo.nii.ac.jp/oai

oai:fra.repo.nii.ac.jp:02002140 2025-03-28T07:31:59Z 15

Aureispira anguillae sp. nov., isolated from Japanese eel Anguilla japonica leptocephali 10392896 Yuasa, Kei 湯浅, 啓 ユアサ, ケイ Yuasa 湯浅 ユアサ Kei 啓 ケイ 40597944 Mekata, Toru 米加田, 徹 メカタ, トオル Mekata 米加田 メカタ Toru 徹 トオル 20443351 Kiryu, Ikunari 桐生, 郁也 キリュウ, イクナリ Kiryu 桐生 キリュウ Ikunari 郁也 イクナリ 90372044 Nomura, Kazuharu 野村, 和晴 ノムラ, カズハル Nomura 野村 ノムラ Kazuharu 和晴 カズハル 60722676 Sudo, Ryusuke 須藤, 竜介 スドウ, リュウスケ Sudo 須藤 スドウ Ryusuke 竜介 リュウスケ 00344325 Satomi, Masataka 里見, 正隆 サトミ, マサタカ Satomi 里見 サトミ Masataka 正隆 マサタカ Aureispira anguillae sp. Nov; Arachidonic acid; Pathogenic marine bacteria; Leptocephali; Japanese eel; Anguilla japonica A novel filamentous eel-leptocephalus pathogenic marine bacterium, designated strain EL160426T, was isolated from Japanese eel, Anguilla japonica, leptocephali reared at a laboratory in Mie, Japan. In experimental infection studies on eel larvae, the strain EL160426T caused massive larval mortality and was reisolated from moribund leptocephali. Characteristically, observations of infected larvae found that EL160426T forms columnar colonies on the cranial surface of larvae. The novel isolate exhibited growth at 15–30 °C, pH 7–9, and seawater concentrations of 60–150% (W/V). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain EL160426T was most closely related to Aureispira maritima 59SAT with 97.7% sequence similarity. The whole genome sequence analysis of the strain EL160426T showed that the strain maintained a circular chromosome with a size of approximately 7.58 Mbp and the DNA G + C content was 36.2%. The major respiratory quinone was MK-7 and the predominant cellular fatty acids were 16:0, 20:4 w6c (arachidonic acid), 17:0 iso and 16:0 N alcohol. DNA relatedness between the closest phylogenetic neighbor strain EL160426T and A. maritima (JCM23207T) was less than 13%. On the basis of the polyphasic taxonomic data, the strain represents a novel species of the genus Aureispira, for which the name Aureispira anguillae sp. nov. is proposed. The type strain is EL160426T (= JCM 35024 T = TSD-286 T). Springer 2023-12-30 eng journal article VoR https://fra.repo.nii.ac.jp/records/2002140 10.1007/s00203-023-03771-x 23310008 水産技術研究所 企画調整部門 （宮古） https://link.springer.com/article/10.1007/s00203-023-03771-x SPRINGER LINK 1432-072X 0302-8933 Archives of Microbiology 206 47 https://fra.repo.nii.ac.jp/record/2002140/files/Yuasa et al. Aureispira anguillae sp. nov.,.pdf application/pdf 1.3 MB 2024-07-03