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Mass mortality events associated with cyprinid herpesvirus 2 (CyHV-2) infection in wild Prussian carp Carassius gibelio in the Netherlands, and molecular biology of virus strains 40934774 0000-0003-3906-1989 Kurobe, Tomofumi 黒部, 智史 クロベ, トモフミ Kurobe 黒部 クロベ Tomofumi 智史 トモフミ 20372039 Kurita, Jun 栗田, 潤 クリタ, ジュン Kurita 栗田 クリタ Jun 潤 ジュン Haenen, Olga Voorbergen-Laarman, Michal 70372050 Ito, Takafumi 伊東, 尚史 イトウ, タカフミ Ito 伊東 イトウ Takafumi 尚史 タカフミ CyHV-2; Prussian carp; cyprinid herpesvirus 2; goldfish; mass mortality events; natural waters In 2011 and 2015, four mass mortalities of Prussian carp (Carassius gibelio) were observed in a recreational freshwater lake and open freshwater in the western part of the Netherlands. Cyprinid herpesvirus 2 (CyHV-2) infection was suspected in these cases, based on presumptive gross diagnosis. To elucidate the cause of the mass mortalities diagnostic PCR assays were performed for CyHV-2, based on the helicase gene. Furthermore, the viral isolates were genotyped by sequencing the enlarged marker A and marker B sequences. Diagnostic PCR revealed that three of four samples were positive for CyHV-2, indicating these three mass mortalities were associated with CyHV-2 infection. The marker A sequence from one of the isolates found in this study was identical to those from different locations such as Asia and Middle East, suggesting a link among the isolates. This is the first detailed report on mass mortalities of Prussian carp associated with CyHV-2 infection in natural aquatic environments in the Netherlands. Since 2015, additionally, in total three CyHV-2 associated outbreaks of Dutch Prussian carp were seen in 2016 and 2020. These outbreaks in Prussian carp from lakes and open water suggest that the virus has been spreading in natural freshwaters in the Netherlands. Wiley 2024-01 eng journal article VoR https://fra.repo.nii.ac.jp/records/2002151 37795684 Pub Med 10.1111/jfd.13868 23336019 水産技術研究所　養殖部門　病理部 （南勢） https://onlinelibrary.wiley.com/doi/10.1111/jfd.13868 WILEY : Online Library 1365-2761 0140-7775 Journal of Fish Diseases 47 1 e13868 https://fra.repo.nii.ac.jp/record/2002151/files/Journal of Fish Diseases - 2023 - Kurobe - Mass mortality events associated with cyprinid herpesvirus 2 CyHV‐2 infection.pdf application/pdf 2.9 MB 2024-07-05