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魚類の胚発生におけるオートファジーが関与する卵黄吸収機構 Autophagy-Mediated Yolk Absorption during Fish Embryogenesis 80510007 Imamura, Shintaro 今村, 伸太朗 イマムラ, シンタロウ Imamura 今村 イマムラ Shintaro 伸太朗 シンタロウ 00551756 Yabu, Takeshi 藪, 健史 ヤブ, タケシ Yabu 藪 ヤブ Takeshi 健史 タケシ 80344323 Yamashita, Michiaki 山下, 倫明 ヤマシタ, ミチアキ Yamashita 山下 ヤマシタ Michiaki 倫明 ミチアキ open access Yolk absorption; proteolysis; autophagy; ubiquitin-proteasome pathway; zebrafi sh; microtubule-associated protein 1 light chain 3 （MAP1LC3） Proteolytic degradation of yolk proteins is essential process for embryogenesis， homeostasis in fish. Since two different major pathways，i. e. ubiquitin-proteasome system and autophagy， have been well known in the animal cells， their roles for the yolk protein proteolysis during embryogenesis were characterized in the embryos of zebrafish and other fish species. Ubiquitin-proteasome system regulates ATP-dependent protein degradation specific to ubiquitinated proteins by proteasome， while autophagy regulates a process of bulky protein degradation via lysosomal/vacuolar system. The proteolytic processing of yolk protein in fish embryogenesis has been identified to be undertaken by lysosomal proteases. In order to characterize the molecular mechanisms on the proteolytic degradation of yolk protein， we examined roles of autophagy during embryogenesis. We established transgenic zebrafish lines， which are expressed a maker protein of autophagosome， microtubule-associated protein 1 light chain 3 （MAP1LC3） fused to green fluorescent protein （GFP）， to visualize autophagosome formed by autophagy. The transgenic embryos were very useful to observe autophagic cells in embryos， and we detected the induction of autophagosome during early embryogenesis. The treatment of embryos with protease inhibitors， such as E-64 （inhibitor of cathepsins B and L） and proteasome inhibitor， effectively repressed yolk protein degradation and embryogenesis， suggesting that both autophagy and proteasome pathways are essential for degradation of yolk proteins. 水産総合研究センター 2008-12 jpn departmental bulletin paper VoR https://fra.repo.nii.ac.jp/records/2010870 fra_k_26_29 https://agriknowledge.affrc.go.jp/RN/2010770088 日本農学文献記事索引（AgriKnowledge） AA11589591 1346-9894 水産総合研究センター研究報告 Bulletin of Fisheries Research Agency 26 6 29 34 https://fra.repo.nii.ac.jp/record/2010870/files/fra_k_26_29.pdf application/pdf 839.5 KB 2024-10-02