2026-03-07T05:16:10Z https://fra.repo.nii.ac.jp/oai

oai:fra.repo.nii.ac.jp:02001694 2025-12-11T00:52:41Z 12:14:1716950587014

超低温下におけるコンブ目植物培養細胞の生存について Survival of cultured cells of a laminariales plant at a super-low temperature 坂西, 芳彦 サカニシ, ヨシヒコ Sakanishi, Yoshihiko 嵯峨, 直恒 サガ, ナオツネ Saga, Naotsune Male gametophytic cells of Macrocystis pyrifera were frozen and preserved in liquid nitrogen. The suspension containing the organisms was cooled at a slow rate ( < 1℃ min-1) to -40℃ in a deep freezer with a polystyrene box, and then, immersed in liquid nitrogen. The cryoprotectant mixture used consisted of 10% (v/v) dimethylsulphoxide and 0.5 M sorbitol based on autoclaved seawater. After storage in liquid nitrogen for 5 days, the cell suspension was rapidly thawed in a water bath at 40˚C, and then, the post-thaw cell viability was checked using the vital-staining method. The post-thaw viability was 45% and recovery growth of cell fragments was confirmed. This is the first report on survival of cultured cells preserved at a super-low temperature in brown algae. departmental bulletin paper 北海道区水産研究所 Hokkaido National Fisheries Research Institute 1992-03 北海道区水産研究所研究報告 56 55 59 Bulletin of the Hokkaido National Fisheries Research Institute AN1015410X 0513-2541 https://fra.repo.nii.ac.jp/records/2001694 jpn hnf_k_56_55 日本農学文献記事索引(agriknowledge) https://agriknowledge.affrc.go.jp/RN/2010501393 metadata only access