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oai:fra.repo.nii.ac.jp:02011921 2026-02-10T02:05:03Z 1:6:1724312740562

マダイ好中球の形態学的および細胞化学的特徴 Morphological and Cytochemical Characteristics of Neutrophil from Red Sea-bream, Pagrus major Kondo, Masakazu 1252 近藤, 昌和 70399666 コンドウ, マサカズ Sakaguchi, Takasuke 3110 坂口, 隆亮 サカグチ, タカスケ Kanamaru, Shunsuke 3026 金丸, 俊介 カナマル, シュンスケ Kashiwamura, Naohiro 3055 柏村, 直宏 カシワムラ, ナオヒロ Takahashi, Yukinori 2815 高橋, 幸則 タカハシ, ユキノリ neutrophil. granulocyte; red sea-bream; Pagrus major; morphology Morphological and cytochemical characteristics of neutrophil in red sea-bream, Pagrus major were examined by light microscopy. The neutrophils were round to oval (7.5-11.5 μm in diameter) and the nucleus round to kidney-shaped. Granules of the neutrophil were classified into two types ; acidophilic granule (αG) and chromophobic granule (βG). The αG was round to oval (0.4μm in diameter) and stained with May-Grunwald (MG) and MG-Giemsa (MGG) stain. The Giemsa staining pattern of the granule was influenced by pH and concentration of diluent of the staining solution. Some enzymes, such as acid phosphatase, α-naphtyl acetate esterase and naphthol AS-D chloroacetate esterase were detected in the αG. The βG was round to oval (≦0.5 u m in diameter) and unstained by Romanowsky type stain (MG, Giemsa and MGG). This granule was peroxidase and sudan black B positive. The Yasumoto body (Y-body) was also found in the neutrophil and toluidine blue positive. departmental bulletin paper 水産大学校 National Fisheries University 2009-11-30 application/pdf 水産大学校研究報告 1 58 15 22 Journal of National Fisheries University AN00124678 0370-9361 https://fra.repo.nii.ac.jp/record/2011921/files/fishu_k_58_15.pdf https://fra.repo.nii.ac.jp/records/2011921 jpn fishu_k_58_15 agriknowledge https://agriknowledge.affrc.go.jp/RN/2010792848 open access