{"created":"2024-04-08T04:47:31.862830+00:00","id":2001691,"links":{},"metadata":{"_buckets":{"deposit":"e909a9f5-defb-4205-b1dd-a51005849958"},"_deposit":{"created_by":10,"id":"2001691","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2001691"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02001691","sets":["12:14:1716950587014"]},"author_link":[],"control_number":"2001691","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992-03","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"10","bibliographicPageEnd":"26","bibliographicPageStart":"17","bibliographicVolumeNumber":"56","bibliographic_titles":[{"bibliographic_title":"北海道区水産研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Hokkaido National Fisheries Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The present study describes the development of an immunodot-blotting technique to determine the sex of Pacific halibut. This method is based on the immunochemical detection of serum vitellogenin existing specifically in females undergoing vitellogenesis. Serum vitellogenin was purified from an estrogen-treated immature females using high-performance liquid chromatography. Two procedures of isolation were combined : gel filtration and anion exchanged chromatography. Isolated vitellogenin has a molecular weight of 380,000 in native form with glycolipophosphoprotein characteristics. Antiserum was raised against estrogen-treated serum, and then, absorbed with male serum to react specifically to estrogen induced proteins including vitellogenin. The developed immunodot-blotting technique by using specific antiserum was effective and rapid determining the sex of Pacific halibut. The evaluated lower limit of this system attained 20ng vitellogenin per dot.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"北海道区水産研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"Hokkaido National Fisheries Research Institute","subitem_publisher_language":"en"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"hnf_k_56_3","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引(agriknowledge)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010501390","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN1015410X","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0513-2541","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松原, 孝博","creatorNameLang":"ja"},{"creatorName":"マツバラ, タカヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Matsubara, Takahiro","creatorNameLang":"en"}],"familyNames":[{"familyName":"松原","familyNameLang":"ja"},{"familyName":"マツバラ","familyNameLang":"ja-Kana"},{"familyName":"Matsubara","familyNameLang":"en"}],"givenNames":[{"givenName":"孝博","givenNameLang":"ja"},{"givenName":"タカヒロ","givenNameLang":"ja-Kana"},{"givenName":"Takahiro","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"澤野, 敬一","creatorNameLang":"ja"},{"creatorName":"サワノ, ケイイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Sawano, Keiichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"澤野","familyNameLang":"ja"},{"familyName":"サワノ","familyNameLang":"ja-Kana"},{"familyName":"Sawano","familyNameLang":"en"}],"givenNames":[{"givenName":"敬一","givenNameLang":"ja"},{"givenName":"ケイイチ","givenNameLang":"ja-Kana"},{"givenName":"Keiichi","givenNameLang":"en"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ビテロジェニンを指標としたドットブロッティングによるオヒョウ (Hippoglossus stenolepis Schmidt) の雌雄判別法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ビテロジェニンを指標としたドットブロッティングによるオヒョウ (Hippoglossus stenolepis Schmidt) の雌雄判別法","subitem_title_language":"ja"},{"subitem_title":"Sex determination of Pacific halibut (Hippoglossus stenolepis Schmidt) by the immunodot-blotting technique using antiserum against vitellogenin","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716950587014"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-04-08"},"publish_date":"2024-04-08","publish_status":"0","recid":"2001691","relation_version_is_last":true,"title":["ビテロジェニンを指標としたドットブロッティングによるオヒョウ (Hippoglossus stenolepis Schmidt) の雌雄判別法"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-01-20T02:14:11.752074+00:00"}