{"created":"2024-04-08T04:48:11.384154+00:00","id":2001718,"links":{},"metadata":{"_buckets":{"deposit":"865cba96-432a-495f-b6b5-fed11bf52d6e"},"_deposit":{"created_by":10,"id":"2001718","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2001718"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02001718","sets":["12:14:1716950587014"]},"author_link":[],"control_number":"2001718","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1990-03","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"6","bibliographicPageEnd":"56","bibliographicPageStart":"51","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"北海道区水産研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Hokkaido National Fisheries Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Various staining-dyes were tested with protoplasts of Ulva pertusa and Enteromorpha intestinalis in order to develop a rapid, simple and reliable technique for determining algal protoplast viability. Protoplasts isolated from unialgal culture strains of the organisms were immersed in the cell viability checking kit and observed with a microscope after 20 min. The most suitable dyes tested were fluorescein diacetate for detection of the viable protoplasts and phenosafranin for detection of the inviable protoplasts in both species.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"プロトプラストは植物細胞に細胞工学や遺伝子操作を施すとき必須の実験系である。プロトプラストの生存率を正確かつ簡便に評価することは、海藻のバイオテクノロジー研究の種々の局面において必要な技術である。海産緑藻アナアオサ及びボウアオノリの単藻培養体からプロトプラストを調製し、数種の染色剤の入ったマイクロプレートCVC-kit(細胞生存率評価キット)を適用して有効な染色剤を調べた。その結果、生細胞の検出にはニュートラルレッドやフルオレセインジアセテートが、死細胞の検出にはフェノサフラニンがもっとも有効であり、大型海藻のプロトプラストの生存率をCVC-kit を用いて簡易に評価できることが解った。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"北海道区水産研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"Hokkaido National Fisheries Research Institute","subitem_publisher_language":"en"}]},"item_10002_radio_30":{"attribute_name":"作成者","attribute_value_mlt":[{"subitem_radio_item":["吉","田"],"subitem_radio_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"hnf_k_54_5","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引(agriknowledge)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010460901","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN1015410X","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0513-2541","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"嵯峨, 直恒","creatorNameLang":"ja"},{"creatorName":"サガ, ナオツネ","creatorNameLang":"ja-Kana"},{"creatorName":"Saga, Naotsune","creatorNameLang":"en"}],"familyNames":[{"familyName":"嵯峨","familyNameLang":"ja"},{"familyName":"サガ","familyNameLang":"ja-Kana"},{"familyName":"Saga","familyNameLang":"en"}],"givenNames":[{"givenName":"直恒","givenNameLang":"ja"},{"givenName":"ナオツネ","givenNameLang":"ja-Kana"},{"givenName":"Naotsune","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"石川, 豊","creatorNameLang":"ja"},{"creatorName":"イシカワ, ユタカ","creatorNameLang":"ja-Kana"},{"creatorName":"Ishikawa, Yutaka","creatorNameLang":"en"}],"familyNames":[{"familyName":"石川","familyNameLang":"ja"},{"familyName":"イシカワ","familyNameLang":"ja-Kana"},{"familyName":"Ishikawa","familyNameLang":"en"}],"givenNames":[{"givenName":"豊","givenNameLang":"ja"},{"givenName":"ユタカ","givenNameLang":"ja-Kana"},{"givenName":"Yutaka","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"坂西, 芳彦","creatorNameLang":"ja"},{"creatorName":"サカニシ, ヨシヒコ","creatorNameLang":"ja-Kana"},{"creatorName":"Sakanishi, Yoshihiko","creatorNameLang":"en"}],"familyNames":[{"familyName":"坂西","familyNameLang":"ja"},{"familyName":"サカニシ","familyNameLang":"ja-Kana"},{"familyName":"Sakanishi","familyNameLang":"en"}],"givenNames":[{"givenName":"芳彦","givenNameLang":"ja"},{"givenName":"ヨシヒコ","givenNameLang":"ja-Kana"},{"givenName":"Yoshihiko","givenNameLang":"en"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"海産緑藻プロトプラストの染色法による生存率決定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"海産緑藻プロトプラストの染色法による生存率決定","subitem_title_language":"ja"},{"subitem_title":"The use of staining-dyes for viability determinations of protoplasts in marine green algae","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716950587014"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-04-08"},"publish_date":"2024-04-08","publish_status":"0","recid":"2001718","relation_version_is_last":true,"title":["海産緑藻プロトプラストの染色法による生存率決定"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-01-20T05:22:51.960333+00:00"}