{"created":"2024-04-23T01:53:23.295024+00:00","id":2002462,"links":{},"metadata":{"_buckets":{"deposit":"42e0fefa-f4bd-40dc-aa16-68ad9c610370"},"_deposit":{"created_by":10,"id":"2002462","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2002462"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02002462","sets":["12:14:1716951426151"]},"author_link":["1966","1873"],"control_number":"2002462","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-03","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"8","bibliographicPageEnd":"138","bibliographicPageStart":"131","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"日本海区水産研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Japan Sea National Fisheries Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA typing of ribosomal RNA genes was performed with a PCR-single strand conformation polymorphism (SSCP) assay for fish identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The product of twelve taxa of fishes (Rajiformes, Salmoniformes, Myctophiformes, Cypriniformes, Anguilliformes, Gasterosteiformes, Syngnathiformes, Gadiformes, Perciformes, Scorpaeniformes, Pleuronectiformes and Tetraodontiformes) classified in order levels were denatured to a single strand DNA and electrophoresed in a non-denaturing polyacrylamide gel. Electrophoretic mobility patterns differed among order levels of fishes, respectively. They indicate the possibility of application of this typing to species identification, using small DNA samples such as an egg or a larva.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"魚類血液から抽出したDNAを材料としてPCR法によりDNAの増幅を試み,その変異性を調べ,種判別法への応用の可能性を検討した.1今回,PCR法で増幅した領域は,28SリボソームRNAをコードする遺伝子の塩基配列から設計·合成されたプライマーであるが,多くの魚類においてうまく増幅することができた.2このことは,魚類においても28SリボソームDNA領域が存在し,広い生物種間で共通性の高い領域であることが確かめられた.328SリボソームDNA領域の変異解析では,目間レベルの魚種判別が可能であった.4極めて微量なDNAで分析が可能であったことから,卵や稚仔など形態からの同定の難しい個体における,新しい魚種判別法の開発の可能性が示された.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本海区水産研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"Japan Sea National Fisheries Research 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