{"created":"2024-04-23T01:53:50.069569+00:00","id":2002471,"links":{},"metadata":{"_buckets":{"deposit":"a47983e4-62d4-4cfc-bf73-f5785dd1895c"},"_deposit":{"created_by":10,"id":"2002471","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2002471"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02002471","sets":["12:14:1716951426151"]},"author_link":["1873"],"control_number":"2002471","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-03","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"7","bibliographicPageEnd":"123","bibliographicPageStart":"117","bibliographicVolumeNumber":"43","bibliographic_titles":[{"bibliographic_title":"日本海区水産研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Japan Sea National Fisheries Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA-fingerprinting with non-radioactive probe was applied to individual identifications in a flounder, Paralichthys olivaceus. (1) A human genome-deviced minisatellite, called myoprobe, could be used for DNA-fingerprinting of a flounder. (2) DNA for analysis of DNA-fingerprinting were extracted from as little as 0.1 ml blood of a flounder or less. (3) Individuals in a flounder were performed by means of DNA-fingerprinting with non-radioactive probe. (4) The DNA-fingerprinting of a flounder which was established on this experiment does not need the radioactive substance as marker attached to the probe. Therefore, it is considered that this method is applied in many laboratories without radio isotope equipment.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"魚類における非放射性プローブを用いたDNAフィンガープリント法を確立するため,ヒトのミオプローブを用いてヒラメのDNAフィンガープリントの検出方法を検討した.1.ヒラメでは非常に微量な血液からゲノムDNAの抽出が可能であった.2.ヒトのミオプローブはヒラメのDNAフィンガープリント法にも使用でき,非常に応用範囲の広いことがわかった.3.非放射性プローブを用いたDNAフィンガープリント法はヒラメの個体識別やクローン解析に応用できることがわかった.4.今回のDNAフィンガープリント法は放射性物質を使用しないことから,RI の特別な施設を持たない場所での使用が可能である.","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本海区水産研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"Japan Sea National Fisheries Research Institute","subitem_publisher_language":"en"}]},"item_10002_radio_30":{"attribute_name":"作成者","attribute_value_mlt":[{"subitem_radio_item":["吉","田"],"subitem_radio_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"js_k_43_117_123","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引(agriknowledge)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010501894","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00186380","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-4620","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata 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Haruo","creatorNameLang":"en"}],"familyNames":[{"familyName":"山内","familyNameLang":"ja"},{"familyName":"ヤマノウチ","familyNameLang":"ja-Kana"},{"familyName":"Yamanouti","familyNameLang":"en"}],"givenNames":[{"givenName":"春夫","givenNameLang":"ja"},{"givenName":"ハルオ","givenNameLang":"ja-Kana"},{"givenName":"Haruo","givenNameLang":"en"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA-Fingerprinting; non-Radioactive; myoprobe; flounder","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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