| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-05-02 |
| タイトル |
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タイトル |
DNA metabarcoding focused on difficult-to-culture protists: An effective approach to clarify biological interactions |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
仲村, 康秀
| en |
Nakamura, Yasuhide
Shimane University
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| ja |
仲村, 康秀
島根大学
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| ja-Kana |
ナカムラ, ヤスヒデ
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Search repository
板垣, ひより
| en |
Itagaki, Hiryori
The University of Tokyo
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| ja |
板垣, ひより
東京大学
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| ja-Kana |
イタガキ, ヒヨリ
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Search repository
辻, 彰洋
| en |
Tuji, Akihiro
National Museum of Nature and Science
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| ja |
辻, 彰洋
国立科学博物館
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| ja-Kana |
ツジ, アキヒロ
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Search repository
下出, 信次
| en |
Shimode, Shinji
Yokohama National University
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| ja |
下出, 信次
横浜国立大学
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| ja-Kana |
シモデ, シンジ
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Search repository
山口, 篤
| en |
Yamaguchi, Atsushi
Hokkaido University
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| ja |
山口, 篤
北海道大学
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| ja-Kana |
ヤマグチ, アツシ
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Search repository
日髙, 清隆
小木曽-田中, 映里
| en |
Ogiso-Tanaka, Eri
National Museum of Nature and Science
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| ja |
小木曽-田中, 映里
国立科学博物館
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| ja-Kana |
オギソ-タナカ, エリ
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Search repository
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
DNA metabarcoding on a single organism is a promising approach to clarify the biological interactions (e.g., predator–prey relationships and symbiosis, including parasitism) of difficult-to-culture protists. To evaluate the effectiveness of this method, Radiolaria and Phaeodaria, which are ecologically important protistan groups, were chosen as target taxa. DNA metabarcoding on a single organism focused on the V9 region of the 18S rRNA gene revealed potential symbionts, parasites and food sources of Radiolaria and Phaeodaria. Previously reported hosts and symbionts (parasites) were detected, and newly recognized combinations were also identified. The contained organisms largely differed between Radiolaria and Phaeodaria. In Radiolaria, members of the same order tended to contain similar organisms, and the taxonomic composition of possible symbionts, parasites, and food sources was fixed at the species level. Members of the same phaeodarian family, however, did not contain similar organisms, and body part (i.e., the central capsule or the phaeodium) was the most important factor that divided the taxonomic composition of detected organisms, implying that the selection of appropriate body part is important when trying to ascertain contained organisms, even for unicellular zooplankton. Our results show that DNA metabarcoding on a single organism is effective in revealing the biological interactions of difficult-to-culture protists. |
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言語 |
en |
| 書誌情報 |
en : Environmental Microbiology
巻 25,
号 12,
p. 3630-3638,
ページ数 9,
発行日 2023-12-19
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| 出版者 |
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出版者 |
Wiley |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1462-2912 |
| 書誌レコードID |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1462-2920 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/1462-2920.16524 |
| 情報源 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
Local |
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関連識別子 |
23235006 |
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言語 |
ja |
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関連名称 |
水産資源研究所 水産資源研究センター 海洋環境部 |
| 関連サイト |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
URI |
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関連識別子 |
https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.16524 |
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言語 |
en |
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関連名称 |
WILEY : Environmental Microbiology |
| 著者版フラグ |
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出版タイプ |
AO |
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出版タイプResource |
http://purl.org/coar/version/c_b1a7d7d4d402bcce |
Nakamura et al Siongle cell DNA metabarcording.pdf (2.4 MB)
