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サケ科魚類におけるナリジクス酸系合成抗菌剤の代謝に関する分析方法の検討
https://fra.repo.nii.ac.jp/records/2007541
https://fra.repo.nii.ac.jp/records/200754145004cd4-cacf-4986-a9a0-9cfc5ef63fd4
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-06-18 | |||||
| タイトル | ||||||
| タイトル | サケ科魚類におけるナリジクス酸系合成抗菌剤の代謝に関する分析方法の検討 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | TLC Analysis of Nalidixic Acid Type Synthetic Antibacterial Agents and Their Metabolites in Salmonids | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | salmonids; nalidixic acid type agents; metabolites | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
石田, 典子
× 石田, 典子× 原, 武史 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Effects of solvent systems and activation of plate were studied on TLC separation of nalidixic acid (NA), oxolinic acid (OA), piromidic acid (PA), unconjugated (α-OHPA, β-OHPA) and conjugated metabolites of these drugs in rainbow trout. Drugs were introduced into rainbow trout orally. Biles were withdrawn 24 hr later, while urines were collected for 48 hr. After extraction of sample fluids with chloroform, unconjugated and conjugated substances were separated into chloroform and aqueous layer, respectively. TLC of unconjugated substances were examined using both standard and chloroform layer of extracts, and TLC of conjugated drugs using aqueous layer. Six acidic solvent systems including n-BuOH or CHCl₃ systems, a neutral solvent system and a basic solvent system were examined. Among the solvent systems examined n-BuOH-AcOH-H₂O (4: 1: 1) produced the best separation for NA and OA, and CHCI₃-formic acid-ethyl formate (8: 1: 4) for PA, α-OHPA and β-OHPA in chloroform layer, respectively. For the sepatation of conjugated NA, OA and PA in aqueous layer n-BuOH-AcOH-H₂O (4: 1: 1) was found to be the most efficient. Little difference of Rf value was observed between the separation of NA and OA using activated and unactivated plate. However, better sepatation was obtained for PA, α-OHPA and β-OHPA using unactivated plate in high humidity of developing chamber. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 養殖研究所研究報告 en : Bulletin of National Research Institute of Aquaculture 巻 7, p. 97-104, ページ数 8, 発行日 1985-03 |
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| 出版者 | ||||||
| 出版者 | 養殖研究所 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0389-5858 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00245945 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | nria_k_7_97 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010320666 | |||||
| 言語 | ja | |||||
| 関連名称 | 日本農学文献記事索引(agriknowledge) | |||||
