{"created":"2024-06-18T03:48:41.163555+00:00","id":2007604,"links":{},"metadata":{"_buckets":{"deposit":"0d20d213-5653-4455-bb02-0d56b3aada7b"},"_deposit":{"created_by":10,"id":"2007604","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2007604"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02007604","sets":["12:14:1716955633551"]},"author_link":[],"control_number":"2007604","item_10002_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-11","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"11","bibliographicPageEnd":"99","bibliographicPageStart":"89","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"養殖研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of National Research Institute of Aquaculture","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Mitochondrial (mt-)DNA was isolated from the oocytes of the Japanese oyster, Crassostrea gigas, and a clone for the linearized full-length mt-DNA was obtained by inserting it into the BamHI site of a plasmid vector, pUC118. The total length of the mt-DNA was 17,700 ± 300 base pairs. A primary restriction map has been derived for the 16 cleavage sites made by 8 enzymes, allowing to serve as the basis for the studies of restriction fragment length polymorphism, DNA sequencing and hybridization detection.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"マガキ(Crassostrea gigas)の未受精卵からミトコンドリア(mt-)DNAを単離し,制限酵素BamHIで線状化した後,プラスミドベクターpUC 118 に挿入して,mt-DNA全長を含む組換え体のクローンを得た。マガキmt-DNAの長さは17,700±300塩基対であった。8つの制限酵素による16の切断箇所について,概略的な制限酵素切断マップを作成した。得られたマップは,制限酵素切断長多型の研究や DNA 塩基配列の決定,およびハイブリダイゼーションによるmt-DNA断片の検出·同定に有用である。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"養殖研究所","subitem_publisher_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"nria_k_16_89","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引(agriknowledge)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010450244","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"item_10002_source_id_11","attribute_value_mlt":[{"subitem_source_identifier":"AN00245945","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"item_10002_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"0389-5858","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大原, 一郎","creatorNameLang":"ja"}],"familyNames":[{"familyName":"大原","familyNameLang":"ja"}],"givenNames":[{"givenName":"一郎","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"森, 勝義","creatorNameLang":"ja"}],"familyNames":[{"familyName":"森","familyNameLang":"ja"}],"givenNames":[{"givenName":"勝義","givenNameLang":"ja"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Crassostrea gigas; mitochondrial DNA; molecular cloning; restriction fragment length polymorphism (RFLP); restriction map","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マガキ(Crassostrea gigas)ミトコンドリアDNAのクローニングとその特徴","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マガキ(Crassostrea gigas)ミトコンドリアDNAのクローニングとその特徴","subitem_title_language":"ja"},{"subitem_title":"Cloning and Characterization of Mitochondrial DNA from the Japanese Oyster, Crassostrea gigas","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716955633551"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-18"},"publish_date":"2024-06-18","publish_status":"0","recid":"2007604","relation_version_is_last":true,"title":["マガキ(Crassostrea gigas)ミトコンドリアDNAのクローニングとその特徴"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-04-02T07:15:21.606781+00:00"}