{"created":"2024-06-18T03:50:01.486824+00:00","id":2007659,"links":{},"metadata":{"_buckets":{"deposit":"7e7c81f6-1164-4bef-9a0b-3c87dffe344b"},"_deposit":{"created_by":10,"id":"2007659","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2007659"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02007659","sets":["12:14:1716955633551"]},"author_link":[],"control_number":"2007659","item_10002_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-03","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"13","bibliographicPageEnd":"25","bibliographicPageStart":"13","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"養殖研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of National Research Institute of Aquaculture","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cryopreservation of the marine microalga Tetraselmis tetrathele (NRIA strain), which is widely used in the aquaculture industry as a food organism, by the two-step freezing method was attempted. A solution composed of 10 to 15% dimethyl sulfoxide (DMSO) and 0.5 M sorbitol in 50% seawater had the favorable cryoprotective effect on cells of the strain. The cells from an exponential phase of growth were found to be sensitive to stresses of freezing and thawing. In contrast, cells from a stationary phase of growth were resistant to these stresses. The most appropriate cryopreservation procedure was as follows: DMSO was added gradually over a period of 15 min followed by an equilibration period of 45 min. The cells were then prefrozen to -40°C at 0.1~1.0°C/min prior to immersion in LN2 (Liquid Nitrogen). After storage in LN2, the cell suspension was thawed quickly by agitation of the vial in a water bath at 40°C, and the cryoprotectants were washed off by gradual dilution with seawater. The survival rate determined by vital staining with erythrosin was about 90%. The cell was independent of the period of storage at least 210 days.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"養殖研究所","subitem_publisher_language":"ja"}]},"item_10002_radio_30":{"attribute_name":"item_10002_radio_30","attribute_value_mlt":[{"subitem_radio_item":"吉田","subitem_radio_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"nria_k_26_13","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引(agriknowledge)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010552830","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"item_10002_source_id_11","attribute_value_mlt":[{"subitem_source_identifier":"AN00245945","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"item_10002_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"0389-5858","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"寺内, 栄一","creatorNameLang":"ja"}],"familyNames":[{"familyName":"寺内","familyNameLang":"ja"}],"givenNames":[{"givenName":"栄一","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"桑野, 和可","creatorNameLang":"ja"}],"familyNames":[{"familyName":"桑野","familyNameLang":"ja"}],"givenNames":[{"givenName":"和可","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"岡内, 正典","creatorNameLang":"ja"}],"familyNames":[{"familyName":"岡内","familyNameLang":"ja"}],"givenNames":[{"givenName":"正典","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"嵯峨, 直恆","creatorNameLang":"ja"}],"familyNames":[{"familyName":"嵯峨","familyNameLang":"ja"}],"givenNames":[{"givenName":"直恆","givenNameLang":"ja"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Chlorophyta; cryopreservation; culture collection; food organism; Tetraselmis tetrathele","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"テトラセルミスTetraselmis tetrathele(緑色植物門，プラシノ藻綱)の凍結保存法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"テトラセルミスTetraselmis tetrathele(緑色植物門，プラシノ藻綱)の凍結保存法の開発","subitem_title_language":"ja"},{"subitem_title":"A Method for Cryopreservation of the Tetraselmis tetrathele (Prasinophyceae, Chlorophyta)","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716955633551"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-18"},"publish_date":"2024-06-18","publish_status":"0","recid":"2007659","relation_version_is_last":true,"title":["テトラセルミスTetraselmis tetrathele(緑色植物門，プラシノ藻綱)の凍結保存法の開発"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-04-04T07:30:14.189766+00:00"}