{"created":"2024-06-18T03:50:18.794999+00:00","id":2007671,"links":{},"metadata":{"_buckets":{"deposit":"b12508aa-eb62-465f-9794-0a0b935859d6"},"_deposit":{"created_by":10,"id":"2007671","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2007671"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02007671","sets":["12:14:1716955633551"]},"author_link":["1999"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-11","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"52","bibliographicPageEnd":"90","bibliographicPageStart":"39","bibliographicVolumeNumber":"28","bibliographic_titles":[{"bibliographic_title":"養殖研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of National Research Institute of Aquaculture","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Preservation of seaweed beds is one of the most important factors for right management of coastal environment in Japan. Many seaweed beds are going to diminish or extinct recently due to \"Iso-yake\" (seaweed withering phenomenon on beach) or development of coastal area and contamination of seawater. It is an urgent issue to preserve or raise healthy seaweed beds. As a basic information to assess at seaweeds fitness, interactions between macroalgae and epiphytic bacteria should be considered. This study was done to clarify the relationship between a brown alga Eisenia bicyclis and its epiphytic bacterial communities, in stress with roles of algal excreted organic matters (EOM). E. bicyclis is a perennial macroalga distributed along the central Pacific coast of Honshu, Japan, and forms a dense marine forest on the rocky bottom. The results obtained are summarized as follows : SEM observation revealed that adhering cocci were dominated on blade surfaces of E. bicyclis. Ratios of respiratory active cells among these cocci were 32-60% on new blade surfaces where bacterial densities were low, and alomost 100% on old blade surfaces where bacterial densities were high. The ratio of respiratory active cells became lower when a blade was incubated under light condition for several hours. The excretion of phenolic substances, which have antimicrobial activities, from E.bicyclis was larger from younger blade and it was also accelerated under light condition. From these results, it is suggested that activities of epiphytic bacteria on E. bicyclis blade surfaces were suppressed by excreted antibacterial substances such as phenols from the algae. Epiphytic bacterial communities on new blade surfaces of E. bicyclis needed algal EOM for their growth. A bacterial strain Moraxella sp. was isolated from a new blade surface of E. bicyclis and the effects of growth stimulation by EOM on this bacterium were examined. EOM included some substances which stimulated the bacterial growth in a small amount, simultaneously with other organic nutrients utilized by bacteria. The substance was thought to be a polysaccharide and its minimum effective concentration was 0.3 μgC/ml as a dissolved organic carbon. The bacterium also could produce extracellular polysaccharide from mannitol, which is a major primary product of photosynthesis of brown algae. Extracellular polysaccharide usually promotes attachment abilities of bacteria. These results indicate that bacteria who colonized to blade surfaces of E. bicyclis early have a strong relationship with substrate macroalgae through algal excreted materials. Alginate degrading bacterial communities occured on old blade surfaces of E. bicyclis were inhibited their alginate degrading activities by algal EOM. Some alginate degrading bacteria isolated from the old blade surfaces of E. bicyclis were inhibited their alginate degrading enzymatic activities but growth. This inhibition by EOM varies depending on the algal blade condition. EOM from healthy blades inhibited alginate degrading activity of these bacteria. On the other hand, the EOM from decomposing algal blades stimulated bacterial activities contrary. These results suggest that decomposition of algae by epiphytic bacteria was controlled by substrate macroalgae through algal excreted materials.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"日本の沿岸環境を維持管理していく上で藻場は重要な要素である。しかし,近年「磯焼け」や沿岸域の開発,海水の汚染等によると考えられる藻場の衰退や消滅がみられ,健全な藻場の維持,育成が急務となっている。そのために必要な基礎的知見のひとっとして,本研究では海藻と葉状体付着細菌との関係に着目した。中部日本の沿岸岩礁域の藻場の主要な構成種の一つである大型多年生の褐藻アラメを材料に用い,海藻の分泌有機物による付着細菌群集の制御機構を明らかにすることを目的とした。得られた結果の要約を以下に示す。アラメの側葉上の細菌相を直接観察すると,固着性の球菌が多くみられた。これらの細菌群集の中で呼吸活性を持つものの割合は,新しい側葉で32~60%であったのに対して,古い側葉ではほぱ100%であり,細菌の付着密度と呼吸活性を持っ細菌の割合の間には相関が見られた。また,新しい側葉を明暗条件下で培養すると,明条件下では細菌の呼吸活性が1.5%まで低下した。同時に測定したアラメの主要な抗菌成分であるフェノールの分泌量は新しい側葉で多く、また明条件下で多かったことから,側葉上の細菌の活性はアラメの分泌する抗菌物質によって影響されていることが推測された。新しい側葉上の細菌群集は,アラメの分泌物を増殖に必要としていた。この細菌群集から得られた細菌株Moraxella sp.を用いて,細菌の増殖に対する分泌物の影響を調べた結果,有機炭素源として利用される成分の他に,微量で細菌の増殖を促進する成分が存在することが示された。この物質は多糖類だと思われ,その最小有効濃度は溶存有機炭素量にして約0.3μgC/mlであった。また,この細菌は褐藻の光合成一次産物であり分泌物中にも多く含まれると予想されるマンニトールを利用して,細胞外多糖を生産する能力を持っていた。古い側葉上のアルギン酸分解細菌群は,アラメ分泌物によってアルギン酸分解酵素活性を抑制されていた。このアルギン酸分解活性抑制効果は分泌物の性質によって異なり,アラメ側葉の活性が低下しているときに放出される物質は逆に細菌によるアルギン酸分解を促進した。以上のようにアラメの側葉上の細菌は,藻体外分泌物から抗菌的な作用を受ける一方で同時に分泌物を有機物源として利用しており,各細菌が増殖や基質利用性といった活性を調節しながら側葉上に生息していることが示された。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"養殖研究所","subitem_publisher_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"nria_k_28_39","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引(agriknowledge)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010590395","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00245945","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0389-5858","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sakami, Tomoko","creatorNameLang":"en","creatorNameType":"Organizational"},{"creatorName":"坂見, 知子","creatorNameLang":"ja","creatorNameType":"Organizational"},{"creatorName":"サカミ, トモコ","creatorNameLang":"ja-Kana","creatorNameType":"Organizational"}],"familyNames":[{"familyName":"Sakami","familyNameLang":"en"},{"familyName":"坂見","familyNameLang":"ja"},{"familyName":"サカミ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Tomoko","givenNameLang":"en"},{"givenName":"知子","givenNameLang":"ja"},{"givenName":"トモコ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1999","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70372034","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70372034"}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"epiphytic bacteria; excreted organic matter; Eisenia bicyclis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"褐藻アラメの側葉上付着細菌に対する藻体外分泌物の影響に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"褐藻アラメの側葉上付着細菌に対する藻体外分泌物の影響に関する研究","subitem_title_language":"ja"},{"subitem_title":"Effects of algal excreted matters on epiphytic bacteria on the brown alga, Eisenia bicyclis","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716955633551"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-18"},"publish_date":"2024-06-18","publish_status":"0","recid":"2007671","relation_version_is_last":true,"title":["褐藻アラメの側葉上付着細菌に対する藻体外分泌物の影響に関する研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2026-02-03T06:58:03.848022+00:00"}