{"created":"2024-06-20T09:03:31.613177+00:00","id":2008146,"links":{},"metadata":{"_buckets":{"deposit":"b27681d1-64c4-410c-bc6a-89c3f6bf69d1"},"_deposit":{"created_by":10,"id":"2008146","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2008146"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02008146","sets":["12:14:1716951205967"]},"author_link":["613","3425"],"control_number":"2008146","item_10002_biblio_info_7":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-12","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"5","bibliographicPageEnd":"59","bibliographicPageStart":"55","bibliographicVolumeNumber":"75","bibliographic_titles":[{"bibliographic_title":"西海区水産研究所研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the Seikai National Fisheries Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Various preculturing conditions were studied in order to improve the survival rates of conchocelis cells of Porphyra yezoensis form. narawaensis after freeze-thaw cycle. The preculturing conditions tested were combinations of temperature (6℃ or 18℃), period (1 or 3 days) and with or without cryoprotectant (0.5 M sorbitol, 0.9 M sorbitol or 0.5M DMSO). The precultured cells were then treated with the cryoprotective solution containing 1.5M DMSO and 0.5 M sorbitol, cooled at 1℃/ min to -45℃ and plunged into liquid nitrogen. After the freeze-thaw cycle, the survival rates of the precultured cells were determined. It was found that preculturing cells with 0.5 M sorbitol, 0.9 M sorbitol or 0.5 M DMSO at 6℃ for 3 days and with 0.5 M sorbitol at 18°C for 1 day improved the survival rate as much as 20% compared with the cells precultured without cryoprotectant at 18℃.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"西海区水産研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"Seikai National Fisheries Research Institute","subitem_publisher_language":"en"}]},"item_10002_radio_30":{"attribute_name":"item_10002_radio_30","attribute_value_mlt":[{"subitem_radio_item":"吉田","subitem_radio_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"snf_k_75_55","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引(agriknowledge)"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010570985","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"item_10002_source_id_11","attribute_value_mlt":[{"subitem_source_identifier":"AN00393385","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"item_10002_source_id_9","attribute_value_mlt":[{"subitem_source_identifier":"0582-415X","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Fujiyoshi, Eiji","creatorNameLang":"en"},{"creatorName":"藤吉, 栄次","creatorNameLang":"ja"},{"creatorName":"フジヨシ, エイジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Fujiyoshi","familyNameLang":"en"},{"familyName":"藤吉","familyNameLang":"ja"},{"familyName":"フジヨシ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Eiji","givenNameLang":"en"},{"givenName":"栄次","givenNameLang":"ja"},{"givenName":"エイジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"613","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80372001","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80372001"}]},{"creatorNames":[{"creatorName":"Umezawa, Satoshi","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"梅澤, 敏","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"ウメザワ, サトシ","creatorNameLang":"ja-Kana","creatorNameType":"Personal"}],"familyNames":[{"familyName":"Umezawa","familyNameLang":"en"},{"familyName":"梅澤","familyNameLang":"ja"},{"familyName":"ウメザワ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Satoshi","givenNameLang":"en"},{"givenName":"敏","givenNameLang":"ja"},{"givenName":"サトシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3425","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ナラワスサビノリ糸状体の超低温保存における前培養条件の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ナラワスサビノリ糸状体の超低温保存における前培養条件の検討","subitem_title_language":"ja"},{"subitem_title":"Preculturing Conditions for Cryopreservation of Conchocelis of Porphyra yezoensis form. narawaensis","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716951205967"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-20"},"publish_date":"2024-06-20","publish_status":"0","recid":"2008146","relation_version_is_last":true,"title":["ナラワスサビノリ糸状体の超低温保存における前培養条件の検討"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-06-05T02:26:09.738271+00:00"}