{"created":"2024-06-27T02:04:47.192328+00:00","id":2009032,"links":{},"metadata":{"_buckets":{"deposit":"7e815158-d4d0-4473-bc92-2e00c12ff3dd"},"_deposit":{"created_by":10,"id":"2009032","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2009032"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02009032","sets":["12:14:1716955633551"]},"author_link":["3282"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1966-11-05","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"15","bibliographicPageEnd":"1297","bibliographicPageStart":"1283","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"国立真珠研究所報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the National Pearl Research Laboratory","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1) 粘液細胞の分布状態と数は外套の内外面あるいは部分によって異なり，生物種ごとに特徴づけられている。\n2) 今回の観察に使用したすべての種類において，外套の内外面にある粘液細胞の内容は酸性ムコ多糖類を含んでいると思われるが，メタクロマジーの強さは生物種によっても，また外套の内外面でも著しく異なっていることがある。生化学的分析と比較すると，Toluidine blue のpH2.1 および 3.2 に対してメタクロマジーを呈した粘液物質は少なくとも硫酸基をもった酸性ムコ多糖類を含んでいると考えられる。\n3) 粘液細胞の内容は分泌されて，extrapallial fluid の１成分となる。両者は染色性と組織化学的反応でよく類似しており，蛋白，PAS陽性物質，酸性ムコ多糖類を含む。\n4) 貝殻の鉱物化層のコンキオリンは extrapallial fluid の有機物質と類似した染色性と組織化学的反応を示すが，反応の強さは両者でかなり異なり，コンキオリンの形成と鉱物化の過程で酸性ムコ多糖類のための反応は弱くなる。\n5) ウミギク，ハナイタヤ，アコヤガイにおいて，殻皮溝中に形成された若い殻皮は強いメタクロマジーを呈したが，他の全種類のそれは Toluidine blue の各pHにたいして全く染まらないか，わずかに青緑に染まる程度でメタクロマジーを呈さなかった。\n6) 軟体動物の石灰化組織の鉱物化機構を討論した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"1) Histochemical investigations were made on mucous cells of the mantle of some molluscs (Gastropoda and Bivalvia), young periostracum formed in periostracal groove, and the extrapallial fluid between the mantle and shell valve. \n2) The distribution and number of mucous cells which show a marked difference between the inner and shell sides, or various areas of a mantle, appear to have taxonomic significance (Figs.1 and 2). \n3) In all species used in the present observation, mucous substance in the mucous cells on the inner and shell mantle surfaces seems to contain acid mucopolysaccharides, one of which appears to be sulfated mucopolysaccharide in the substances metachromatically stained with pH 2.1 and 3.4 of toluidine blue, but the intensity of metachromasia varies according to molluscan species and local area of a mantle (Tables 2 and 3). \n4) Secreted mucous substances are incorporated in the extrapallial fluid between the mantle and shell valve (Figs. 5,6,7 and 8). The staining and histochemical properties of the organic substances in the fluid agree well with those of the mucous substance in the cells (Figs. 5,6,7 and 8). \n5) The organic substances in the extrapallial fluid are similar to the organic matrix \"conchiolin\" of mineralized layers of a shell in staining and histochemical reactions. However, changes of the intensity of those reactions indicate that acid mucopolysaccharides in the organic substances may decrease but are not lost in the process of formation and mineralization of conchiolin (Table 5). Biochemical investigations by Tanaka and Hatano (1963), Wada (in press), and Furuhashi and Wada (in press) also suggest that a part of the organic substances in the fluid may be lost, later proteins concentrate in the shell matrix during those processes. \n6) In Spondulus barbatus, Pecten (Notovola) laqueatus, and Pinctada martensii, young periostracum formed between the shell and middle folds of a mantle shows metachromasia (Table 4), but that of all other species used in this study is unstained or slightly greenish blue in colour when stained with toluidine blue.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立真珠研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"National Pearl Research Laboratory","subitem_publisher_language":"en"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"pearl_k_1283","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本真珠振興会Archive"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://jp-pearl.com/wp-content/uploads/2018/06/houkoku011.pdf#001","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00091717","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wada, Koji","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"和田, 浩爾","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"ワダ, コウジ","creatorNameLang":"ja-Kana","creatorNameType":"Personal"}],"familyNames":[{"familyName":"Wada","familyNameLang":"en"},{"familyName":"和田","familyNameLang":"ja"},{"familyName":"ワダ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Koji","givenNameLang":"en"},{"givenName":"浩爾","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3282","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"軟体動物の石灰化組織の鉱物化に関する研究　13. 外套における粘液細胞の分布状態とその内容の組織化学的研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"軟体動物の石灰化組織の鉱物化に関する研究　13. 外套における粘液細胞の分布状態とその内容の組織化学的研究","subitem_title_language":"ja"},{"subitem_title":"Studies on the mineralization -of the calcified tissue in molluscs. XIII. Histological and histochemical studies of mucous cells on the inner and shell surfaces of mantle of some bivalve and gastropod molluscs ..1283","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716955633551"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-27"},"publish_date":"2024-06-27","publish_status":"0","recid":"2009032","relation_version_is_last":true,"title":["軟体動物の石灰化組織の鉱物化に関する研究　13. 外套における粘液細胞の分布状態とその内容の組織化学的研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-02-13T02:43:21.372066+00:00"}