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水槽飼育アコヤガイの成長におよぼす各種緩衝試薬の影響について
https://fra.repo.nii.ac.jp/records/2009051
https://fra.repo.nii.ac.jp/records/20090513de24d33-f8b6-46cf-8e38-15807dd93d99
| アイテムタイプ | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2024-06-27 | |||||
| タイトル | ||||||
| タイトル | 水槽飼育アコヤガイの成長におよぼす各種緩衝試薬の影響について | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Effects on the growth of Japanese pearl oyster of the various alkaline reagents added to the culture water for the control of its pH | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
桑谷, 幸正
× 桑谷, 幸正× 西飯, 保 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 閉鎖循環式飼育装置7組を用い,各組に満1年生アコヤガイ5個ずつを収容し,それぞれの飼育水にそれらのpH値が常に8.0以上になるように各種の緩衝試薬を添加し,無添加のものを対象として56日間の飼育期間における水質および貝の成長の変化を調査して,それぞれの試薬の緩衝効果および貝に与える影響について検討した。得られた結果はつぎのとおりである。 1) pH8.0以上になるように添加した各試薬の0.1N溶液の総添加量はトリス‐buffer,ベロナールナトリウムがとくに多く,以下ホウ酸ナトリウム,水酸化ナトリウム,水酸化カルシウム,炭酸ナトリウムの順であった。各飼育水の期間中の平均pH値は炭酸ナトリウム,ベロナールナトリウム,ホウ酸ナトリム,水酸化カルシウム,水酸化ナトリウム,対照,トリス‐buffer の添加水の順であった。トリス‐buffer 添加水は35日目頃からpHの上昇がみられず,45日目からその添加を中止した。期間中のpH値の変化から判断すると,水酸化ナトリウムの添加量は飼育水のpH値を調製するのに充分でなかったと思われる。対照を除くその他の4飼育水のpH値には明らかな差異は認められない。 2) 炭酸分圧はベロナールナトリウムとトリス‐buffer 添加水とが最も高く,ついでホウ酸ナトリウム添加水が高く,その他は対照と同様に低い値を示した。重炭酸イオンおよび炭酸イオン濃度はトリス‐buffer を除き炭酸分圧と同様の傾向を示した。トリス‐buffer 添加水は重炭酸イオン濃度においてはベロナールナトリウムとホウ酸ナトリウム添加水との中間に位置し,炭酸イオン濃度においては35日目まではホウ酸ナトリウム添加水のそれと同様の値を,それ以後においてはかなりの低減を示した。 3) 窒素系3態についてはいずれの値においてもトリス―buffer 添加水が他の飼育水にくらべ著しく高い。 4) 各貝群はその成長量の順につぎの4群に分けられる。 (1)ホウ酸ナトリウム,ベロナールナトリウム添加群 (2)水酸化カルシウム,炭酸ナトリウム添加群 (3)水酸化ナトリウム,トリス‐buffer 添加群 (4)対照群 以上の結果からつぎのことが議論される。 1) いずれの試薬添加群の成長も対照群のそれより良好であったことは,炭酸分圧の増大にかかわらず,pH値の回復が貝の成長にとってより好条件であったことを示している。 2) 長期間の無換水飼育の場合に,トリス‐buffer の添加は窒素系の増大をもたらし,直接あるいは間接的に水質を悪化する要因となる。 3) ホウ酸ナトリウム,ベロナールナトリウム(上記第1群)および水酸化カルシウム,水酸化カルシウム(上記第2群)の4試薬添加による飼育水のpH値の差はほとんどなく,また炭酸分圧はむしろ第1群の両飼育水が高く,上記のような貝の成長のちがいは試薬の質的な差とみることができる。上記第1群の両飼育水に用いた試薬は他の試薬にくらべ,いずれもその解離度が著しく低いのが注目される。 |
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| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The present study has been done to make clear the effects on the growth of the Japanese pearl oyster, Pinctada fucata(Gould), of various alkaline reagents added to the culture water for the control of its pH. Thirty five one-year-old pearl oysters were equally divided into 7 groups, which were reared in separate recirculating tanks for 56 days. All the groups were given rice powder as food at a rate of 40 mg per oyster per day. In order to keep the pH of culture water above 8.0 any of six kinds of reagents was added. Chemical analysis of culture water and measurements of underwater weight of the animals were made for each experimental group every seven days, and the careful inspection was then made on the buffer effects of the alkaline reagents used and also the effects of these reagents on the growth of pearl oyster. The results obtained are summarized as follows: 1) Tris-buffer or barbital sodium required by far the greatest amount to maintain the pH value of culture water above 8.0, and they were followed by sodium borate, sodium hydroxide, calcium hydroxide and then sodium carbonate. As for the average pH values of culture water the greatest figure was obtained in association with sodium carbonate addition, and next in descending order are barbital sodium, sodium borate, calcium hydroxide, sodium hydroxide, control and Tris-buffer addition. The addition of Tris-buffer was discontinued 45 days after the begining of experiment, because, in spite of its addition, the pH value of culture water never increased from the 35th day onward. No remarkable differences were observed in the pH values among the use of the above four reagents. 2) The partial pressure of carbon dioxide was the highest in the culture water added barbital sodium or Tris-buffer, and the second highest in that added sodium borate, while the remaining reagents gave as low values as control. As for the concentrations of bicarbonate and carbonate ions of culture water the same tendency as in the case of the partial pressure of carbon dioxide was exhibited in the culture water recived the alkaline reagents except for Tris-buffer. The concentration of carbonate ions in the Tris-buffer-added culture water kept the same level as that in sodium borate-added water during the first 35 days of experiment, but it fell down considerably thereafter 3) The Tris-buffer-added culture water exhibited a remarkably high level for ammonia, nitrite and nitrate as compared with the case of the other alkaline reagents. 4) From the cumulative growth patterns of pearl oyster reared it was possible to class the seven differently treated groups of oyster as follows: a) Sodium borate and barbital sodium oyster groups; they showed the most rapid growth throughout the rearing period. b) Calcium hydroxide and sodium carbonate oyster groups; they showed a tendency that the growth, though it was slow, might continue even after the experiment. c) Sodium hydroxide and Tris-buffer oyster groups; they showed a decline in weight on the way of rearing. d) Control oyster group; they showed a negative growth in weight except for at the very beginning of the experiment. From the results obtained the following discussions are made: 1) With the result that the growth of any of treated oyster groups was better than that of the control oyster group, it can be said that the artificial control of pH of the culture water by alkaline reagents created a favorable condition for the growth of pearl oyster, even under the condition that oysters were exposed to the increasing partial pressure of carbon dioxide. 2) When oysters were reared without changing water for a long period of time, the continued addition of Tris-buffer may be a cause of water deterioration, bringing the increase of ammonia, nitrite and nitrate, and the falling down of pH value associated with the increase of nitrate exerted unfavorable influence upon the growth of the animals. 3) In spite of the facts that there was no significant difference in pH level between the (a) and (b) growth groups mentioned before, and that the partial pressure of carbon dioxide was higher in the first group than in the second group, the growth of pearl oyster in the first group surpassed that in the second group. Therefore, the difference in growth between the above two groups was possibly due to the different nature of the reagents applied. Here, it is worthy of note that the reagents which brought about the most rapid growth of oyster possess a considerably low dissociation degree. |
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| 言語 | en | |||||
| 書誌情報 |
ja : 国立真珠研究所報告 en : Bulletin of the National Pearl Research Laboratory 巻 13, p. 1597-1616, ページ数 20, 発行日 1968-07-05 |
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| 出版者 | ||||||
| 出版者 | 国立真珠研究所 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | National Pearl Research Laboratory | |||||
| 言語 | en | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00091717 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | pearl_k_1597 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://jp-pearl.com/wp-content/uploads/2018/06/houkoku013.pdf#004 | |||||
| 言語 | ja | |||||
| 関連名称 | 日本真珠振興会Archive | |||||