{"created":"2024-06-27T02:05:55.116861+00:00","id":2009076,"links":{},"metadata":{"_buckets":{"deposit":"615f04af-bad9-4ffc-aa20-71a66729a692"},"_deposit":{"created_by":10,"id":"2009076","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2009076"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02009076","sets":["12:14:1716955633551"]},"author_link":["3282"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1973-08-25","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"16","bibliographicPageEnd":"2074","bibliographicPageStart":"2059","bibliographicVolumeNumber":"17","bibliographic_titles":[{"bibliographic_title":"国立真珠研究所報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of the National Pearl Research Laboratory","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1) アコヤガイ，ムラサキイガイ，マガキ，およびヒオウギの外套膜の連続クリオスタット切片を作り，外套膜組織における alkaline phosphatase，acid phosphatase，succinate dehydrogenase，lactate dehydrogenase，および NADH dehydrogenase の分布と活性の程度を組織化学的に調べた。\n2) Alkaline phosphatase と adid phosphatase の反応は外套膜上皮細胞にかぎられて認められ，acid phosphatase の活性は alkaline phosphatase の活性よりも相対的には強かった。\n3) Alkaline phosphatase の活性は上皮細胞の遠心端に巾狭く認められ，外套膜上の位置による酵素活性の程度は貝の種類によってかなり異なる。\n4) 貝殻形成に直接関与する上皮細胞の acid phosphatase の活性は細胞の遠心側原形質で強く，本酵素は少なくとも細胞内消化に関連していると考えられる。\n5) Succinate dehydrogenase，lactate dehydrogenase，および NADH dehydrogenase の強い反応が外套膜の上皮細胞，特に貝殻に面した，に認められた。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"The localization and activity of various enzymes in the mantle tissue of Pinctada fucata, Mytilus edulis, Crassostrea gigas and Chlamys nobilis were investigated by histochemical methods. \nFresh frozen sections were prepared in the cryostat at-20℃. The demonstration of alkaline phosphatase (Burstone, 1962), acid phosphatase (Burstone, 1962), succinate dehydrogenase (Barka and Anderson, 1963), lactate dehydrogenase (Nachlas et al., 1958) and NADH dehydrogenase (Barka and Anderson, 1963) was carried out following the methods shown in Table 1. \nAlkaline and acid phosphatases are present only in the epithelium of mantle tissue of the marine bivalves, though the degree of activity differs remarkably between species and even between parts of a mantle. Strong activity of alkaline phosphatase is found in the distal end of the epithelial cell (Figs. 2 B, C), and acid phosphatase activity is high in the distal cytoplasm. \nThe activity of such oxidative enzymes as succinate, lactate and NADH dehydrogenases tends to be much higher in the epithelium of outer mantle surface than in the epithelium of inner mantle surface (Figs. 4, 5 and 6), in all species examined. \nThe results obtained in the present investigation are roughly summarized in Table 2.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立真珠研究所","subitem_publisher_language":"ja"},{"subitem_publisher":"National Pearl Research Laboratory","subitem_publisher_language":"en"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"pearl_k_2059","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本真珠振興会Archive"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://jp-pearl.com/wp-content/uploads/2018/07/houkoku017.pdf#002","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00091717","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wada, Koji","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"和田, 浩爾","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"ワダ, コウジ","creatorNameLang":"ja-Kana","creatorNameType":"Personal"}],"familyNames":[{"familyName":"Wada","familyNameLang":"en"},{"familyName":"和田","familyNameLang":"ja"},{"familyName":"ワダ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Koji","givenNameLang":"en"},{"givenName":"浩爾","givenNameLang":"ja"},{"givenName":"コウジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3282","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"外套膜の酵素組織化学による研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"外套膜の酵素組織化学による研究","subitem_title_language":"ja"},{"subitem_title":"Enzyme histochemistry of mantle tissue of some bivalves","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1716955633551"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-06-27"},"publish_date":"2024-06-27","publish_status":"0","recid":"2009076","relation_version_is_last":true,"title":["外套膜の酵素組織化学による研究"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-02-13T02:45:06.664458+00:00"}