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Establishment of cell-lines stably expressing recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone using CHO-DG44 cells: fully induced ovarian development at different modes
https://fra.repo.nii.ac.jp/records/2009839
https://fra.repo.nii.ac.jp/records/2009839cbda361e-fd76-415c-9a10-42d17eeab533
| 名前 / ファイル | ライセンス | アクション |
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Kazeto et al. Establishment of cell-lines stably expressing recombinant Japanese eel.pdf (4.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2024-07-11 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Establishment of cell-lines stably expressing recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone using CHO-DG44 cells: fully induced ovarian development at different modes | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Japanese eel; fecundity; ovarian development; recombinant follicle-stimulating hormone; recombinant luteinizing hormone. | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||
| 著者 |
風藤, 行紀
× 風藤, 行紀× 伊藤, 理沙
× 田中, 寿臣
× 鈴木, 博史× 尾﨑, 雄一× 奥澤, 公一× 玄, 浩一郎 |
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| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | The gonadotropins (Gth), follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh), play central roles in gametogenesis in vertebrates. However, available information on their differential actions in teleost, especially in vivo, is insufficient. In this study, we established stable CHO-DG44 cell lines expressing long-lasting recombinant Japanese eel Fsh and Lh with extra O-glycosylation sites (Fsh-hCTP and Lh-hCTP), which were produced in abundance. Immature female eels received weekly intraperitoneal injections of Gths. Fsh-hCTP induced the entire ovarian development by 8 weeks from the beginning of injection; thus, the ovaries of most fish were at the migratory nucleus stage while the same stage was observed in eels after 4 weeks in the Lh-hCTP-treated group. In contrast, all pretreated and saline-injected eels were in the pre-vitellogenic stage. Gonadosomatic indices in the Fsh-hCTP-treated group were significantly higher than those in the Lh-hCTP group at the migratory nucleus stage because of the significantly higher frequency of advanced ovarian follicles. Ovarian mRNA levels of genes related to E2 production (cyp11a1, cyp17a1, cyp19a1, hsd3b, fshr, and lhr) were measured using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). All genes were induced by both Fsh-hCTP and Lh-hCTP, with a peak at either the mid- or late vitellogenic stages. Transcript abundance of cyp19a1 and fshr in the Lh-hCTP group were significantly higher than those in the Fsh-hCTP group, whereas no difference in the expression of other genes was observed between the groups. Fluctuations in serum levels of sex steroid hormones (estradiol-17β, 11-ketotestosterone, and testosterone) in female eels were comparable in the Fsh-hCTP and Lh-hCTP groups, thus increasing toward the maturational phase. Furthermore, the fecundity of the eels induced to mature by Fsh-hCTP was significantly higher than that induced by Lh-hCTP. These findings indicate that Fsh and Lh can induce ovarian development in distinctively different modes in the Japanese eel. | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 書誌情報 |
en : Frontiers in Endocrinology 巻 14, 号 2023, 発行日 2023-08-25 |
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| 出版者 | ||||||||||||||||||
| 出版者 | Frontiers Media | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||
| 収録物識別子 | 1664-2392 | |||||||||||||||||
| PubMed番号 | ||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||
| 関連識別子 | 37693354 | |||||||||||||||||
| DOI | ||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||
| 関連識別子 | 10.3389/fendo.2023.1201250 | |||||||||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||||||||
| 識別子タイプ | Local | |||||||||||||||||
| 関連識別子 | 23332040 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| 関連名称 | 水産技術研究所 養殖部門 シラスウナギ生産部 | |||||||||||||||||
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| 関連タイプ | isIdenticalTo | |||||||||||||||||
| 識別子タイプ | URI | |||||||||||||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/37693354/ | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 関連名称 | Pub Med | |||||||||||||||||
| 著者版フラグ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
