| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-07-11 |
| タイトル |
|
|
タイトル |
Detection of markers for proliferation, stem cell, and EMT in the gills of Pacific abalone Haliotis discus hannai |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Hemocyte; Gill; Proliferation; Stem cell; Abalone |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
ソデヤマ, ジン
コガ, トウマ
モリ, ユウイチ
イナダマ, リョウタ
モリ, カズマ
オグラ, チカ
キノムラ, アカネ
舩山, 翔平
モリヤマ, シュンスケ
オクムラ, セイイチ
フルカワ, フミヤ
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The hemocytes of molluscs take various roles including immunity and wound healing. Oyster gill was found to undergo hematopoiesis and produce adult somatic progenitor cells. In gastropod molluscs, however, such special gill structure has not been reported. In the present study, we used Pacific abalone, a gastropod mollusc, and explored unique gill structures undergoing active proliferation. By immunofluorescence staining, two proliferation markers, proliferating cell nuclear antigen (PCNA) and phospho-histone H3 (H3P), were detected in marginal regions of the gill filaments, which were characterized by thick epithelium with cilia. In situ hybridization for Sox genes, the stem cell markers, also showed signals in the corresponding regions. BrdU, a DNA replication-detecting reagent, and nuclear-localized β-catenin, a putative epithelial–mesenchymal transition (EMT) marker, were also detected in the same regions. These results suggest that the marginal regions of gill filaments are highly proliferative, possess stemness, and likely undergoing EMT. Further immunofluorescence staining for hemocytes with PCNA, H3P, and β-catenin revealed that these cells also exhibited these markers, implying a possible link between the hemocytes and the gill cells. These results showed a structure containing actively proliferating cells in abalone gills, and points to the importance of classification of cell population in this tissue. |
|
言語 |
en |
| 書誌情報 |
en : Fisheries Science
巻 89,
p. 605-612,
ページ数 8,
発行日 2023-06-15
|
| 出版者 |
|
|
出版者 |
Springer |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0919-9268 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s12562-023-01701-w |
| 情報源 |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
Local |
|
|
関連識別子 |
23334034 |
|
|
言語 |
ja |
|
|
関連名称 |
水産技術研究所 養殖部門 生理機能部 |
| 関連サイト |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://link.springer.com/article/10.1007/s12562-023-01701-w |
|
|
言語 |
en |
|
|
関連名称 |
SPRINGER LINK |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Sodeyama et al. Detection of markers for proliferation.pdf (1.1 MB)
