{"created":"2024-07-22T02:32:03.821115+00:00","id":2010376,"links":{},"metadata":{"_buckets":{"deposit":"29edd532-efd9-48ec-98f2-a30e5cebc762"},"_deposit":{"created_by":92,"id":"2010376","owner":"92","owners":[92],"pid":{"revision_id":0,"type":"depid","value":"2010376"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02010376","sets":["15"]},"author_link":["705"],"control_number":"2010376","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-05-17","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"23","bibliographic_titles":[{"bibliographic_title":"Bulletin of the European Association of Fish Pathologists","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Since the first occurrence of White Spot Disease (WSD) in 1992, it is still listed as one of the crustacean diseases by the World Organisation for Animal Health in 2022. Horizontal transmission in aquaculture systems is considered as a major contributing factor to the spread of the disease. WSD outbreak was recorded during the experimental run of the co-culture of black tiger prawn (Penaeus monodon) and sandfish (Holothuria scabra) in the grow-out phase. In this study, artificial infection through cohabitation experiments were conducted to determine if H. scabra is a potential vector or reservoir of WSSV. During the first cohabitation (5 days) where WSSV-infected prawn were cohabited with WSSV-free sandfish, 100% of the prawn were one step-positive PCR for WSSV while 100% of the sandfish (coelom, intestine, tentacle, and muscle) were nested-positive PCR for WSSV. Subsequently, WSSV-positive H. scabra from the first cohabitation were transferred to another tank to be cohabited with WSSV-free P. monodon. Sampling of both prawn and sandfish was done every six days post-infection (dpi). At 6 dpi, all prawn and only 1 sandfish were nested-positive for WSSV.  At 12 dpi, majority of the prawn were nested-positive and none for sandfish.  At 18 dpi, prawn were all negative and one sandfish was nested-positive. At 25 dpi, there were no prawn left due to mortality and 1 of the 3 remaining sandfish was nested positive for WSSV. Based on the results, it was elucidated that sandfish were able to bioaccumulate the viral particles when cohabited with WSSV-infected prawn. Relative to these findings, it was concluded that WSSV is viable in the sandfish due to the consistent nested-positive results of the prawn during the second cohabitation and the occurrence of mortality in the latter part of the experiment. Sandfish could still be safely used for the next co-culture cropping only after quarantine for at least 1 month since the result showed that muscle and intestine can still be WSSV nested-positive after 25 dpi. Hence, sandfish can be a potential reservoir of WSSV for a limited period of time.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"European Association of Fish Pathologists","subitem_publisher_language":"en"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"水産技術研究所 環境・応用部門 沿岸生態システム部"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"23342256","subitem_relation_type_select":"Local"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"SSRN"}],"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4437867","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0108-0288","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Southeast Asian Fisheries Development Center (SEAFDEC)","affiliationNameLang":"en"},{"affiliationName":"東南アジア漁業開発センター","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Dela Pena, Leobert D.","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Southeast Asian Fisheries Development Center (SEAFDEC)","affiliationNameLang":"en"},{"affiliationName":"東南アジア漁業開発センター","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Caber, Dieyna B.","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Lawrence Berkeley National Laboratory","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Villacastin, Anne Jinky","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Hebrew University of Jerusalem","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Cabillon, Nikko Alvin","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"University of Crete","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Arboleda, Joey","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Southeast Asian Fisheries Development Center (SEAFDEC)","affiliationNameLang":"en"},{"affiliationName":"東南アジア漁業開発センター","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Castellano, Jose Louis Abong","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Watanabe, Satoshi","creatorNameLang":"en"},{"creatorName":"渡部, 諭史","creatorNameLang":"ja"},{"creatorName":"ワタナベ, サトシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Watanabe","familyNameLang":"en"},{"familyName":"渡部","familyNameLang":"ja"},{"familyName":"ワタナベ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Satoshi","givenNameLang":"en"},{"givenName":"諭史","givenNameLang":"ja"},{"givenName":"サトシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"705","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40371877","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40371877"},{"nameIdentifier":"0000-0002-5630-632X","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-5630-632X"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-08-26"}],"filename":"Dela Pena et al sandfish-_holothuria-scabra_-as-potential-reservoir-of-white-spot-syndrome-virus-wssv-when-co-cultured-with-black-tiger-prawn-_penaeus-monodon_.pdf","filesize":[{"value":"293 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2010376/files/Dela Pena et al sandfish-_holothuria-scabra_-as-potential-reservoir-of-white-spot-syndrome-virus-wssv-when-co-cultured-with-black-tiger-prawn-_penaeus-monodon_.pdf"},"version_id":"2764b79d-fa2a-4ce0-bd4f-c3105b9df065"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Sandfish; White spot syndrome virus (WSSV); co-culture; PCR; cohabitation Introduction","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sandfish (Holothuria Scabra) as Potential Reservoir of White Spot Syndrome Virus (Wssv) When Co-Cultured with Black Tiger Prawn (Penaeus Monodon)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sandfish (Holothuria Scabra) as Potential Reservoir of White Spot Syndrome Virus (Wssv) When Co-Cultured with Black Tiger Prawn (Penaeus Monodon)","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"92","path":["15"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-07-22"},"publish_date":"2024-07-22","publish_status":"0","recid":"2010376","relation_version_is_last":true,"title":["Sandfish (Holothuria Scabra) as Potential Reservoir of White Spot Syndrome Virus (Wssv) When Co-Cultured with Black Tiger Prawn (Penaeus Monodon)"],"weko_creator_id":"92","weko_shared_id":-1},"updated":"2025-03-27T07:41:20.542867+00:00"}