{"created":"2024-08-06T09:02:31.090236+00:00","id":2010523,"links":{},"metadata":{"_buckets":{"deposit":"e4a9aec4-b080-48a8-88b9-3d1a19387a4d"},"_deposit":{"created_by":10,"id":"2010523","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2010523"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02010523","sets":["1:7:1723085692591"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicNumberOfPages":"5","bibliographicPageEnd":"139","bibliographicPageStart":"135","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"水産技術","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of fisheries technology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To develop a simple extraction method for ATP and its related compounds from fish muscles for onsite measurements with ATP sensors, we tested different methods of homogenization or extraction solvents. Extractions of ATP related compounds from the skeletal muscles of the horse mackerel （Trachurus japonicas） were carried out by 1） homogenizing with a Polytron homogenizer, 2） homogenizing with a food processor, 3） mincing with a glass rod, and 4） cutting the tissues into cubes of side ca 5 mm. Sufficient amounts of ATP for detection by an ATP sensor were extracted from the small cubes by shaking in 10% PCA. Effectiveness of ATP extraction with various solvents such as water, 3% NaCl aqueous solution, 80% ethanol, 10% TCA, 10% HCl, 20% acetic acid, acetic acid/ethanol/water （10/40/50%, v/v/v%）, and 1% PCA were compared with that with the conventional solvent, 10% PCA. A one percent solution of PCA was able to extract ATP without degradations and yielded an ATP solution at a concentration of 80 μM, higher than the detection-limit by an ATP sensor. Thus it was inferred that ATP and its related compounds can be extracted from tissues cut into small pieces with shaking in 1% PCA.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"ATP センサーを用いて現場で即時に測定を行うための ATP 関連化合物簡易抽出法の開発を目的とし，マアジを用い，使用する筋肉の部位，試料のホモジナイズ方法，抽出溶媒の検討を行った。使用する筋肉の部位により抽出した ATP 関連化合物の組成には有意な差は無かった。また，筋肉片を 5 mm 角程度に細切した後，10 mℓの溶媒中で 20 秒程度振盪するだけで，センサーの検出閾値以上（＞ 10 μM）の ATP が抽出できた。溶媒として 1％過塩素酸を用いることで，ATP が分解されることなく抽出されることが分かった。以上のことから，1％過塩素酸による振盪法が ATP を簡易的に抽出できる方法として有効であった。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産総合研究センター","subitem_publisher_language":"ja"},{"subitem_publisher":"Japan Fisheries Research Agency","subitem_publisher_language":"en"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"fra_t_5_2_135","subitem_relation_type_select":"Local"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引（AgriKnowledge）"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010853328","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12359338","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1883-2253","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"可児, 祥子","creatorNameLang":"ja"}],"familyNames":[{"familyName":"可児","familyNameLang":"ja"}],"givenNames":[{"givenName":"祥子","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"坂口, 明美","creatorNameLang":"ja"}],"familyNames":[{"familyName":"坂口","familyNameLang":"ja"}],"givenNames":[{"givenName":"明美","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"村田, 裕子","creatorNameLang":"ja"}],"familyNames":[{"familyName":"村田","familyNameLang":"ja"}],"givenNames":[{"givenName":"裕子","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"村田, 昌一","creatorNameLang":"ja"}],"familyNames":[{"familyName":"村田","familyNameLang":"ja"}],"givenNames":[{"givenName":"昌一","givenNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-08-06"}],"filename":"fra_t_5_2_135.pdf","format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2010523/files/fra_t_5_2_135.pdf"},"version_id":"9df271ff-547f-4642-a22b-30f2ff314e7c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マアジ筋肉からのATP関連化合物の簡易抽出法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マアジ筋肉からのATP関連化合物の簡易抽出法の検討","subitem_title_language":"ja"},{"subitem_title":"Investigation of a Simple Extraction Method for ATP and Its Related Compounds from the Muscle of Horse Mackerel (Trachurus japonicas)","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["1723085692591"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-08-06"},"publish_date":"2024-08-06","publish_status":"0","recid":"2010523","relation_version_is_last":true,"title":["マアジ筋肉からのATP関連化合物の簡易抽出法の検討"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-09-05T08:35:48.005951+00:00"}