{"created":"2024-08-06T09:03:54.141306+00:00","id":2010534,"links":{},"metadata":{"_buckets":{"deposit":"48a087c9-63f1-4e74-8099-d0c28d352b17"},"_deposit":{"created_by":10,"id":"2010534","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2010534"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02010534","sets":["1:7:1723085692335"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicNumberOfPages":"7","bibliographicPageEnd":"167","bibliographicPageStart":"161","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"水産技術","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of fisheries technology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Several species of dinoflagellate produce paralytic shellfish toxin (PST), the accumulation of which in bivalves is a serious problem to public health and the fisheries and aquaculture of bivalves. The causative substances are saxitoxin and its analogues. The direct measurement of these substances in seawater samples was studied as an alternative method for the monitoring of the causative dinoflagellate Alexandrium tamarense by microscopic observation. Toxin profiles in 23 strains of A. tamarense isolated from Osaka Bay were analyzed by high-performance liquid chromatography (HPLC), and the major toxin in these strains was C2 (N21-sulfocarbamoyl gonyautoxin-3). The S/N (signal to noise ratio) of the C2 peak in HPLC analysis was over the quantitative level (S/N>10) when the cell density of A. tamarense in the sample was >100 cells/mL. Since the prefectural government has called for attention to PST in bivalves when the cell density of A. tamarense exceeds 5 cells/mL, the volume of the sample should be reduced to 1/20 to detect C2 by HPLC for monitoring purposes. In spring 2012, seawater samples were continuously collected at two monitoring sites in Osaka Bay, and the toxin contents and cell density of A. tamarense were analyzed. The toxin contents by direct measurement had good correlations (St. 12: r=0.834; St. 19: r=0.817) with the cell density. These results indicate the possibility of the direct measurement of PST as an alternative method of monitoring.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"海水中の麻痺性貝毒量をモニタリング指標として利用することを目指し，モニタリングで必要とされる感度で毒量を高速液体クロマトグラフィー（HPLC）により測定する方法を検討するとともに，大阪湾の現場海水試料で指標としての可能性を検証した。大阪湾のAlexandrium tamarense は複数含まれる毒成分のうち C2（N21-sulfocarbamoyl gonyautoxin-3）の組成比が最も高く，培養細胞を添加した海水の検討では、行政が注意喚起する細胞密度（5 cells/mL）において、C2 分析ピークの S/N 比（signal to noise ratio）が定量下限の 10 を超えるには，目合い 15μm のネットで 20 倍以上に濃縮することが必要と考えられた。2012 年 2 ～ 5 月に大阪湾で採取した海水は A. tamarense が最大で 1.0 cells/mL と低かったため 100 倍以上の濃縮を行い分析したところ，C2 の測定値と細胞密度は良い相関を示し，本手法による毒量値はモニタリング指標として利用できる可能性があると考えられた。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル名末尾が”_abs_j”のファイルは和文要旨","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産総合研究センター","subitem_publisher_language":"ja"},{"subitem_publisher":"Japan Fisheries Research Agency","subitem_publisher_language":"en"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"fra_t_6_2_161","subitem_relation_type_select":"Local"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引（AgriKnowledge）"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010871227","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12359338","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1883-2253","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"及川, 寛","creatorNameLang":"ja"}],"familyNames":[{"familyName":"及川","familyNameLang":"ja"}],"givenNames":[{"givenName":"寛","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"山本, 圭吾","creatorNameLang":"ja"}],"familyNames":[{"familyName":"山本","familyNameLang":"ja"}],"givenNames":[{"givenName":"圭吾","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"長井, 敏","creatorNameLang":"ja"}],"familyNames":[{"familyName":"長井","familyNameLang":"ja"}],"givenNames":[{"givenName":"敏","givenNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-08-06"}],"filename":"fra_t_6_2_161.pdf","format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2010534/files/fra_t_6_2_161.pdf"},"version_id":"eba1d89e-2302-4a09-8af0-e2fba62bd81b"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-08-06"}],"filename":"fish_t_6_2_161_abs_j.pdf","format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2010534/files/fish_t_6_2_161_abs_j.pdf"},"version_id":"a87a073d-34ed-4141-879b-b3e686d337dc"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"海水試料の毒量分析による麻痺性貝毒モニタリング手法の開発と検証","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"海水試料の毒量分析による麻痺性貝毒モニタリング手法の開発と検証","subitem_title_language":"ja"},{"subitem_title":"Analysis of Paralytic Shellfish Toxin Content in the Water Column as an Altemative Method of Monitoring Alexandrium tamarense","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["1723085692335"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-08-06"},"publish_date":"2024-08-06","publish_status":"0","recid":"2010534","relation_version_is_last":true,"title":["海水試料の毒量分析による麻痺性貝毒モニタリング手法の開発と検証"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-06-10T03:02:19.295947+00:00"}