{"created":"2024-08-06T09:09:27.095178+00:00","id":2010618,"links":{},"metadata":{"_buckets":{"deposit":"19de3e97-01b8-4c8c-a2b0-2ffddc10104c"},"_deposit":{"created_by":10,"id":"2010618","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2010618"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02010618","sets":["1:7:1723007904522"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicNumberOfPages":"7","bibliographicPageEnd":"29","bibliographicPageStart":"23","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"水産技術","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of fisheries technology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Grazing by heterotrophic dinoflagellates often plays an important role in the termination of harmful algal blooms. However, there are no simple methods to detect the ingestion of harmful algae by heterotrophic dinoflagellates in the field. Here, we examined the loop-mediated isothermal amplification (LAMP) method to detect the grazing by Noctiluca scintillans on Karenia mikimotoi and Chattonella spp. in laboratory and field experiments. In the laboratory, the LAMP method detected DNA from K. mikimotoi in N. scintillans food vacuoles for no longer than 3 h after their ingestion, indicating active grazing by N. scintillans in the early diurnal period. In Ago Bay, Japan, we collected N. scintillans at intervals of 2-5 h during August 2015, when K. mikimotoi and Chattonella spp. co-occurred. The LAMP method detected DNA from K. mikimotoi and Chattonella spp. ingested by N. scintillans with high frequency during the daytime. These results show that the LAMP method can detect grazing by N. scintillans on harmful algae, and that daytime sampling is optimal to identify active grazing.","subitem_description_language":"en","subitem_description_type":"Abstract"},{"subitem_description":"ヤコウチュウに摂食された有害赤潮プランクトンのLAMP法による検出法を検討した。まず，本手法を用いた室内実験を行い，ヤコウチュウは摂食した KareniamikimotoiのDNAを3時間以内に消化することや明期前半に活発に摂食することを見出した。また. K. mikimotoi およびChattonellaspp. の赤潮発生時夜間よりも昼間に高い確率でヤコウチュウの細胞内から両種を検出した。以上のことから，結果の解釈に消化や摂食リズムなどを考慮する必要があるが．ヤコウチュウによる有害赤潮プランクトンの摂食解析に LAMP法を適用可能であると考えられた。","subitem_description_language":"ja","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産研究・教育機構","subitem_publisher_language":"ja"},{"subitem_publisher":"Japan Fisheries Research and Education Agency","subitem_publisher_language":"en"}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"fra_t_12_1_23","subitem_relation_type_select":"Local"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"ja","subitem_relation_name_text":"日本農学文献記事索引（AgriKnowledge）"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010931680","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12359338","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1883-2253","subitem_source_identifier_type":"PISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"北辻, さほ","creatorNameLang":"ja"}],"familyNames":[{"familyName":"北辻","familyNameLang":"ja"}],"givenNames":[{"givenName":"さほ","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"紫加田, 知幸","creatorNameLang":"ja"}],"familyNames":[{"familyName":"紫加田","familyNameLang":"ja"}],"givenNames":[{"givenName":"知幸","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"坂本, 節子","creatorNameLang":"ja"}],"familyNames":[{"familyName":"坂本","familyNameLang":"ja"}],"givenNames":[{"givenName":"節子","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"中山, 奈津子","creatorNameLang":"ja"}],"familyNames":[{"familyName":"中山","familyNameLang":"ja"}],"givenNames":[{"givenName":"奈津子","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"永井, 清仁","creatorNameLang":"ja"}],"familyNames":[{"familyName":"永井","familyNameLang":"ja"}],"givenNames":[{"givenName":"清仁","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"鬼塚, 剛","creatorNameLang":"ja"}],"familyNames":[{"familyName":"鬼塚","familyNameLang":"ja"}],"givenNames":[{"givenName":"剛","givenNameLang":"ja"}]},{"creatorNames":[{"creatorName":"多田, 邦尚","creatorNameLang":"ja"}],"familyNames":[{"familyName":"多田","familyNameLang":"ja"}],"givenNames":[{"givenName":"邦尚","givenNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-08-06"}],"filename":"fra_t_12_1_23.pdf","format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2010618/files/fra_t_12_1_23.pdf"},"version_id":"db564eea-b761-4b53-b886-3eb4c0d33cc0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ヤコウチュウ; 有害赤潮プランクトン;  LAMP法; 摂食","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"LAMP法を活用したヤコウチュウに摂食された有害赤潮プランクトンの検出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"LAMP法を活用したヤコウチュウに摂食された有害赤潮プランクトンの検出","subitem_title_language":"ja"},{"subitem_title":"Detection of harmful algae grazed by Noctiluca scintillans by LAMP method","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["1723007904522"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-08-06"},"publish_date":"2024-08-06","publish_status":"0","recid":"2010618","relation_version_is_last":true,"title":["LAMP法を活用したヤコウチュウに摂食された有害赤潮プランクトンの検出"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-09-05T08:42:11.112265+00:00"}