| アイテムタイプ |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2024-09-30 |
| タイトル |
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タイトル |
アワビ類初期生態解明のための種判別技術の開発 |
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言語 |
ja |
| タイトル |
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|
タイトル |
Development of a discrimination methods by using molecular and monoclonal antibodies techniques for Haliotis discus discus, H. madaka and H. gigantea during their early life stages. |
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言語 |
en |
| 言語 |
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言語 |
jpn |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
H. discus discus; H. madaka; H. gigantea; species discrimination; PCRRFLP; monoclonal antibodies |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
浜口, 昌巳
佐々木, 美穂
堀井, 豊充
清本, 節夫
WEKO
481
e-Rad_Researcher
70371995
| en |
Kiyomoto, Setsuo(Personal)
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| ja |
清本, 節夫(Personal)
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| ja-Kana |
キヨモト, セツオ(Personal)
|
Search repository
大橋, 智志
| en |
Ohashi, Satoshi(Personal)
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| ja |
大橋, 智志(Personal)
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| ja-Kana |
オオハシ, サトシ(Personal)
|
Search repository
藤井, 明彦
| en |
Fujii, Akihiko(Personal)
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| ja |
藤井, 明彦(Personal)
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| ja-Kana |
フジイ, アキヒコ(Personal)
|
Search repository
滝口, 直之
| en |
Takiguchi, Naoyuki(Personal)
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| ja |
滝口, 直之(Personal)
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| ja-Kana |
タキグチ, ナオユキ(Personal)
|
Search repository
橋本, 加奈子
竹内, 泰介
| en |
Takeuchi, Taisuke(Personal)
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| ja |
竹内, 泰介(Personal)
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| ja-Kana |
タケウチ, タイスケ(Personal)
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Search repository
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Three species of abalones, Haliotis discus discus, H. madaka and H. gigantea, are distributed along the southwest coast in Japan and are common and commercially important shellfish in the country. Survey records show a rapid decline in fisheries production of abalones in the last decade, however, few attempts have so far been made to study the early life stages of these species. One of the bottleneck problems in the study of early life stages of these species is the difficulty to discriminate between species since their morphological features are very similar. In addition, a hybrid variety of H. discus discus, and H. madaka had been reported and this fact has made the species identification problem more complex. The purpose of this paper was to develop a discrimination method by using molecular and monoclonal antibodies techniques for Haliotis discus discus, H. madaka and H. gigantea during their early life stages. Molecular(PCR)techniques To discriminate between Haliotis discus discus, H. madaka and H. gigantea, we analyzed some mitochondrial, 16S rDNA, COI(cytchrome c oxidase subunit I), Cyt b (cytchrome b)and nuclear genes, 18S and 28S rDNA, ITS(internally transcribed spacer), ferritin, and calmodulin-introns and discovered that PCR products related tocalmodulin-intron were very suitable for species discrimination. A PCR-RFLP(restriction fragment length polymorphisms)method was developed using calmodulinintrons. Fertilized eggs and planktonic larvae were analyzed by using 2 step PCR method. |
|
言語 |
en |
| 書誌情報 |
ja : 水産研究総合センター研究報告別冊
en : Bulletin of Fisheries Research Agency(Supplement)
巻 5,
p. 75-83,
ページ数 9,
発行日 2006-03
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| 出版者 |
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出版者 |
水産総合研究センター |
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言語 |
ja |
| 出版者 |
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出版者 |
Japan Fisheries Research Agency |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1346-9894 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA11589591 |
| 情報源 |
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識別子タイプ |
Local |
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|
関連識別子 |
fra_kb_5_75 |
| 著者版フラグ |
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|
出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 見出し |
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|
大見出し |
2:資源添加等の生態学・分子生物学的解明・評価に基づく放流技術の高度化 |
|
小見出し |
1)アワビサブチーム |