{"created":"2024-10-03T01:22:36.807766+00:00","id":2010873,"links":{},"metadata":{"_buckets":{"deposit":"c0c0cf6a-0ba5-4b87-a82b-09cb242d4ac0"},"_deposit":{"created_by":10,"id":"2010873","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2010873"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02010873","sets":["1:8:1724310835301"]},"author_link":["664","1963"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-12","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"6","bibliographicPageEnd":"52","bibliographicPageStart":"47","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"水産総合研究センター研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of  Fisheries Research Agency","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cytokines are a group of proteins that are involved in a variety of immunological and inflammatory reactions. Recently, it has been reported that cytokine genes also play an important role during development in mammals. In fish, however little is known about the role of immune-related genes during embryogenesis. Here, we report the expression and function of some cytokines during embryonic development in zebrafish. In RT-PCR analysis, the expression of three cytokines (MIF, IL-1 β and TNF- α 2) was detected before the development of the immune system in zebrafish embryos. Moreover, to elucidate the function of MIF during embryonic development in fish, we examined expression patterns and function of the zebrafish MIF gene using antisense morpholino-mediated knockdown (morpholino oligonucleotide-MO). In whole-mount in situ hybridization analysis, zebrafish MIF mRNA was detected in developing eyes, tectum, branchial arches, pectoral fi n buds, liver and gut. MIF-MO-injected embryos (morphants) displayed malformed eyes, abnormal swelling in the tectum and fourth ventricle region, and undeveloped jaw cartilage and pectoral fi ns. An increased number of apoptotic cells in the eye and neural tissues were observed in MIF morphants. These results suggest that MIF is essential for normal embryonic development even at the level of teleost.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産総合研究センター","subitem_publisher_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"fra_k_26_47","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"日本農学文献記事索引（AgriKnowledge）"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010770091","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11589591","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-9894","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Yoshiura, Yasutoshi","creatorNameLang":"en"},{"creatorName":"吉浦, 康寿","creatorNameLang":"ja"},{"creatorName":"ヨシウラ, ヤストシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Yoshiura","familyNameLang":"en"},{"familyName":"吉浦","familyNameLang":"ja"},{"familyName":"ヨシウラ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yasutoshi","givenNameLang":"en"},{"givenName":"康寿","givenNameLang":"ja"},{"givenName":"ヤストシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"664","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90372052","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90372052"}]},{"creatorNames":[{"creatorName":"Ototake, Mitsuru","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"乙竹, 充","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"オトタケ, ミツル","creatorNameLang":"ja-Kana","creatorNameType":"Personal"}],"familyNames":[{"familyName":"Ototake","familyNameLang":"en"},{"familyName":"乙竹","familyNameLang":"ja"},{"familyName":"オトタケ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Mitsuru","givenNameLang":"en"},{"givenName":"充","givenNameLang":"ja"},{"givenName":"ミツル","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1963","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60360765","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60360765"}]},{"creatorNames":[{"creatorName":"Ito, Kanako","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"伊藤, かな子","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"イトウ, カナコ","creatorNameLang":"ja-Kana","creatorNameType":"Personal"}],"familyNames":[{"familyName":"伊藤","familyNameLang":"ja"}],"givenNames":[{"givenName":"かな子","givenNameLang":"ja"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-02"}],"filename":"fra_k_26_47.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2010873/files/fra_k_26_47.pdf"},"version_id":"c1fce8af-740c-4cf8-bb33-dcc03e45e84c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cytokine; Macrophage migration inhibitory factor (MIF); early development; zebrafish; morpholino","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"魚類の初期発生期におけるサイトカイン遺伝子群の機能を探る","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"魚類の初期発生期におけるサイトカイン遺伝子群の機能を探る","subitem_title_language":"ja"},{"subitem_title":"Expression and function of cytokine genes in the early development of fish","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1724310835301"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-02"},"publish_date":"2024-10-02","publish_status":"0","recid":"2010873","relation_version_is_last":true,"title":["魚類の初期発生期におけるサイトカイン遺伝子群の機能を探る"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-03-27T07:18:10.151096+00:00"}