{"created":"2024-10-03T01:28:09.057721+00:00","id":2010932,"links":{},"metadata":{"_buckets":{"deposit":"d3e12077-f9a6-4d57-8b19-3c5b75b176b8"},"_deposit":{"created_by":10,"id":"2010932","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2010932"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02010932","sets":["1:8:1724310831374"]},"author_link":["690","312"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03","bibliographicIssueDateType":"Issued"},"bibliographicNumberOfPages":"8","bibliographicPageEnd":"128","bibliographicPageStart":"121","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"水産総合研究センター研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Bulletin of  Fisheries Research Agency","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In hatcheries the induction of spawning in sea cucumbers has been typically carried out by regulation of rearing conditions such as temperature and light intensity. However, this method is relatively ineffective and the rate of spawning is unpredictable. In this study we established an efficient method for inducing spawning in the Japanese sea cucumber Apostichopus japonicus by injecting a neuropeptide, cubifrin. We purified peptides that can induce oocyte maturation from the buccal tissues containing the nerve ring, using liquid chromatography and in vitro assay with ovarian fragments in combination. The effective dose of each peptide was evaluated with chemically synthesized peptides. Consequently, the most potent peptide was identified as NGIWY-amide. We also found that synthetic derivatives replaced the third amino acid, isoleucine, with a different basic amino acid could be 10-100 times more potent than the natural hormone. When injected into the body cavity of sexually matured individuals, NGIWY-amide, or its derivative, induced spawning in both males and females. NGIWY-amide was then named cubifrin after the Japanese word “kubifuri” meaning waving head, which is a reproductive behavior of sea cucumbers. Gamete shedding started about 60 min and 80 min after the injection in males and females, respectively, and was completed almost simultaneously in the two sexes about 2 hours after the administration. The in vitro responsiveness of biopsied ovarian fragments was well correlated with the spawning success induced by an injection. Therefore, cubifrin can be used as an excellent detector of maturity as well as an inducer of spawning in A. japonicus in a hatchery setting.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産総合研究センター","subitem_publisher_language":"ja"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"fra_k_40_121","subitem_relation_type_select":"Local"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11589591","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-9894","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Yamano, Keisuke","creatorNameLang":"en"},{"creatorName":"山野, 恵祐","creatorNameLang":"ja"},{"creatorName":"ヤマノ, ケイスケ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Yamano","familyNameLang":"en"},{"familyName":"山野","familyNameLang":"ja"},{"familyName":"ヤマノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Keisuke","givenNameLang":"en"},{"givenName":"恵祐","givenNameLang":"ja"},{"givenName":"ケイスケ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"690","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10372020","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10372020"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Fujiwara, Atsushi","creatorNameLang":"en"},{"creatorName":"藤原, 篤志","creatorNameLang":"ja"},{"creatorName":"フジワラ, アツシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Fujiwara","familyNameLang":"en"},{"familyName":"藤原","familyNameLang":"ja"},{"familyName":"フジワラ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Atsushi","givenNameLang":"en"},{"givenName":"篤志","givenNameLang":"ja"},{"givenName":"アツシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"312","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30443352","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30443352"},{"nameIdentifier":"0000-0002-6717-2259","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-6717-2259"}]},{"creatorNames":[{"creatorName":"YOSHIKUNI, Michiyasu","creatorNameLang":"en","creatorNameType":"Personal"}],"familyNames":[{"familyName":"YOSHIKUNI","familyNameLang":"en"}],"givenNames":[{"givenName":"Michiyasu","givenNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-02"}],"filename":"fra_k_40_121.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2010932/files/fra_k_40_121.pdf"},"version_id":"19976b62-9202-4d14-9c63-7093cffcc5b0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"reproduction; hormone; aquaculture; echinoderm","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Induced Spawning in the Sea Cucumber Apostichopus japonicus by Neuropeptide, Cubifrin","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Induced Spawning in the Sea Cucumber Apostichopus japonicus by Neuropeptide, Cubifrin","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1724310831374"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-02"},"publish_date":"2024-10-02","publish_status":"0","recid":"2010932","relation_version_is_last":true,"title":["Induced Spawning in the Sea Cucumber Apostichopus japonicus by Neuropeptide, Cubifrin"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-03-27T07:31:44.649449+00:00"}