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わが国の沿岸海域における炭化水素酸化細菌に関する生態学的研究 (6) 海洋中の重油分解細菌のスクリーニング法
https://fra.repo.nii.ac.jp/records/2011451
https://fra.repo.nii.ac.jp/records/2011451bf433dbd-36e2-4afd-bed1-975a2440b7f9
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-10-11 | |||||
| タイトル | ||||||
| タイトル | わが国の沿岸海域における炭化水素酸化細菌に関する生態学的研究 (6) 海洋中の重油分解細菌のスクリーニング法 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Ecolgical studies on hydrocarborn-oxidizing bacteria in Japanese coastal waters - 6 Screening method of heavy oil-decomposing bacteria in the sea | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
藤沢, 浩明
× 藤沢, 浩明× 村上, 正忠 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The present study was undertaken to find out a useful method for the screening of heavy oil-decomposing bacteria in the area polluted by the Mizushima Oil Refinery Accident, a serious spill of heavy oil (grade C) into the sea. The results obtained can be summarized as follows; A membrane filter method was available for isolating directly heavy oil-decomposing bacteria groups A and B from seawater and bottom sediment. Serial dilutions of bacterial source were filtered through a membrane filter of 0.45 um in pore size. Bacteria caught on the filter were incubated for 14-21 days at 25℃ on agar medium A or B in which heavy oil (grade C) was added as substrate, and colonies of bacteria grown up on the filter were picked up. These bacteria isolated were incubated in liquid medium A or B in which heavy oil was contained, under constant shaking of 110 strokes per minute at 25℃ for 14 days. The remaining hydrocarbons in each culture liquid were separated by silicic acid column chromatography and gravimetrically determined. Each decomposition rate of heavy oil (hydrocarbons) was estimated in comparison with control value. It was recognized by gas chromatography that the greater part of n-paraffin components (from n-pentadecane or n-hexadecane to n-tetracontane) in heavy oil was clearly decomposed after incubation. The heavy oil-decomposing activity of bacteria examined was divided into 6 grades on the basis of decomposition rate of the substrate. As to bacteria isolated from seawater and bottom sediment in Bisan Seto and Yoshimi coastal waters, both 98 strains out of the 118 strains of group A and 140 strains out of the 148 strains of group B possessed clear activity. Especially, 55 strains out of the whole of group A and 103 strains out of the whole group B, ranked with higher three grades. Therefore, it was concluded that the above-mentioned method (isolation of heavy oil-decomposing bacteria and determination of heavy oil-decomposing activity of bacteria isolated), was useful for the screening of heavy oil-decomposing bacteria in the sea. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 水産大学校研究報告 en : The Journal of the Shimonoseki University of Fisheries 巻 30, 号 1, p. 13-24, ページ数 12, 発行日 1981-11 |
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| 出版者 | ||||||
| 出版者 | 水産大学校 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | the Shimonoseki University of Fisheries | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0370-9361 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00124678 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | fishu_k_30_13 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010253632 | |||||
| 言語 | ja | |||||
| 関連名称 | 日本農学文献記事索引(agriknowledge) | |||||
