{"created":"2024-10-15T05:02:49.646572+00:00","id":2011741,"links":{},"metadata":{"_buckets":{"deposit":"ac2a92aa-ebe6-4c8d-a4cc-f473033b95c8"},"_deposit":{"created_by":10,"id":"2011741","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2011741"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02011741","sets":["1:6:1724312742586"]},"author_link":["1252","1254","2815"],"control_number":"2011741","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicNumberOfPages":"9","bibliographicPageEnd":"117","bibliographicPageStart":"109","bibliographicVolumeNumber":"50","bibliographic_titles":[{"bibliographic_title":"水産大学校研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of National Fisheries University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"May-Grünwald . Giemsa staining properties of granules in carp neutrophil were examined in this study. Two types of granules, eosinophilic granule (αG) and chromophobic granule (ßG) were observed in one neutrophil. The αG was small (<0.3m in diameter) and round in shape, and ßG was round to oval and was larger than αG. The distribution pattern of αG was influenced by pH, constituent and concentration of diluent of the staining solution. To estimate the influence of diluent, five neutrophil types were established as follows: type 0, no αG in the cell; type I, αG was observed in less than 10%; typeII, in 10~40%; typeIII, in 40~80%; typeIV, in more than 80% area of cytoplasms. From typeIV to type I, αG disappeared from perinuclear area to epicyte. When distilled water was used as a diluent, pH did not influence on αG staining, but in the case of phosphate buffer, pH was important factor for staining of αG. At low concentration of phosphate buffer, pH did not influence in αG staining. These variations of αG staining may be due to the maturation of the granules. Furthermore, we expected that the granule is constantly secreted from the neutrophil, because the distribution patterns do not relate to nucleus shape.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産大学校","subitem_publisher_language":"ja"},{"subitem_publisher":"National Fisheries 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ユキノリ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Takahashi","familyNameLang":"en"},{"familyName":"高橋","familyNameLang":"ja"},{"familyName":"タカハシ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yukinori","givenNameLang":"en"},{"givenName":"幸則","givenNameLang":"ja"},{"givenName":"ユキノリ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2815","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-11"}],"filename":"fishu_k_50_109.pdf","filesize":[{"value":"1.1 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neutrophil","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1724312742586"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-11"},"publish_date":"2024-10-11","publish_status":"0","recid":"2011741","relation_version_is_last":true,"title":["コイ好中球顆粒のメイ-グリュンワルド・ギムザ染色性"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-04-25T06:02:56.764759+00:00"}