{"created":"2024-10-15T05:03:16.956609+00:00","id":2011748,"links":{},"metadata":{"_buckets":{"deposit":"6a4bd66b-7a47-4768-b177-2d26f96dc85c"},"_deposit":{"created_by":10,"id":"2011748","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2011748"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02011748","sets":["1:6:1724312742343"]},"author_link":["2985","2936"],"control_number":"2011748","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicNumberOfPages":"6","bibliographicPageEnd":"12","bibliographicPageStart":"7","bibliographicVolumeNumber":"51","bibliographic_titles":[{"bibliographic_title":"水産大学校研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of National Fisheries University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Porphyra farming is one of the most important aquaculture in Japan. Ten billion \"Nori sheet\" have been produced every year. However, almost harvested Porphyra have been processed to dried sheet only. It is very difficult to keep a good quality of fresh Porphyra for a long time, even if it was frozen. Because cells in Porphyra laver dead by freezing and successive cryopreservation. Dead laver lost a unique texture and flavor. Therefore, we search for the freezing and cryopreservation conditions to keep a living laver for several months. Two important facts were known to survive the laver cells under freezing and crypreservation process. Firstly, laver must be cooled below 1.0C per minute. Secondly, laver must be cryopreserved with cryoprotective additive such as a 20% D-glucose solution. Both this cooling rate and cryoprotectant were necessary for cryopreservation of living Porphyra laver. A life and death of laver was discriminate easily by the observation of the cells using a microscope. Plasmolysis was observed in the dead cells. Plasmolysis caused by freezing or cryopreservation did not recover after thawing and successive incubation.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産大学校","subitem_publisher_language":"ja"},{"subitem_publisher":"National Fisheries University","subitem_publisher_language":"en"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"fishu_k_51_7","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"agriknowledge"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010673185","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00124678","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0370-9361","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kunimoto, Masahiko","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"国本, 正彦","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"クニモト, マサヒコ","creatorNameLang":"ja-Kana","creatorNameType":"Personal"}],"familyNames":[{"familyName":"Kunimoto","familyNameLang":"en"},{"familyName":"国本","familyNameLang":"ja"},{"familyName":"クニモト","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Masahiko","givenNameLang":"en"},{"givenName":"正彦","givenNameLang":"ja"},{"givenName":"マサヒコ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2936","nameIdentifierScheme":"WEKO"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Kito, Hitoshi","creatorNameLang":"en"},{"creatorName":"鬼頭, 鈞","creatorNameLang":"ja"},{"creatorName":"キトウ, ヒトシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Kito","familyNameLang":"en"},{"familyName":"鬼頭","familyNameLang":"ja"},{"familyName":"キトウ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Hitoshi","givenNameLang":"en"},{"givenName":"鈞","givenNameLang":"ja"},{"givenName":"ヒトシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2985","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-11"}],"filename":"fishu_k_51_7.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2011748/files/fishu_k_51_7.pdf"},"version_id":"9c1dec51-ee0d-4342-a8a3-44020f772c84"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"生ノリ葉体の凍結保存","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"生ノリ葉体の凍結保存","subitem_title_language":"ja"},{"subitem_title":"Cryopreservation of Porphyra laver","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1724312742343"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-11"},"publish_date":"2024-10-11","publish_status":"0","recid":"2011748","relation_version_is_last":true,"title":["生ノリ葉体の凍結保存"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-04-25T06:02:56.229481+00:00"}