{"created":"2024-10-15T05:30:20.921172+00:00","id":2012111,"links":{},"metadata":{"_buckets":{"deposit":"73b5d9a9-13a6-4e36-a315-462cb767f51e"},"_deposit":{"created_by":10,"id":"2012111","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"2012111"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:02012111","sets":["1:6:1724312738447"]},"author_link":["1255"],"control_number":"2012111","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicNumberOfPages":"11","bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"水産大学校研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Journal of National Fisheries University","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Vasa gene homologs of tiger puffer (Takifugu rubripes, Tp-vasa)and grass puffer (T. niphobles, Gp-vasa) were cloned and characterized for use as a molecular marker of germ cells in these species. Analysis of the nucleotide sequence revealed that open reading frames of Tp-and GP-vasa comprise 1,872 bp (encoding 623 amino acids) and 1,869 bp (encoding 622 amino acids), respectively. These deduced amino acid sequences contained arginine—glycine or arginine-glycine-glycine motifs and eight conserved motifs belonging to the DEAD-box protein family, and showed highly similarity and identity with vasa homologs of other teleosts. Tissue specific expressions of Tp- and Gp-vasa were detected in testis and ovary. In situ hybridization analysis showed that Tp- and Gp-vasa mRNA expressed in oogonia and oocytes in the ovary, and spermatogonia in the testis, while it was not detected in the primary and secondary spermatocytes, spermatid, spermatozoa or gonadal somatic cells. These findings suggest that Tp- and Gp-vasa should be useful for studies of germ cells specification and differentiation in Takifugu puffers.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"水産大学校","subitem_publisher_language":"ja"},{"subitem_publisher":"National Fisheries University","subitem_publisher_language":"en"}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"fishu_k_66_1","subitem_relation_type_select":"Local"}}]},"item_10002_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"agriknowledge"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://agriknowledge.affrc.go.jp/RN/2010922179","subitem_relation_type_select":"URI"}}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00124678","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0370-9361","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Yoshikawa, Hiroyuki","creatorNameLang":"en"},{"creatorName":"吉川, 廣幸","creatorNameLang":"ja"},{"creatorName":"ヨシカワ, ヒロユキ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Yoshikawa","familyNameLang":"en"},{"familyName":"吉川","familyNameLang":"ja"},{"familyName":"ヨシカワ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Hiroyuki","givenNameLang":"en"},{"givenName":"廣幸","givenNameLang":"ja"},{"givenName":"ヒロユキ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1255","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40733936","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40733936"},{"nameIdentifier":"0000-0002-6673-7924","nameIdentifierScheme":"ORCID","nameIdentifierURI":"https://orcid.org/0000-0002-6673-7924"}]},{"creatorNames":[{"creatorName":"Ino, Yasuko","creatorNameLang":"en","creatorNameType":"Personal"},{"creatorName":"井野, 靖子","creatorNameLang":"ja","creatorNameType":"Personal"},{"creatorName":"イノ, ヤスコ","creatorNameLang":"ja-Kana","creatorNameType":"Personal"}],"familyNames":[{"familyName":"Ino","familyNameLang":"en"},{"familyName":"井野","familyNameLang":"ja"},{"familyName":"イノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yasuko","givenNameLang":"en"},{"givenName":"靖子","givenNameLang":"ja"},{"givenName":"ヤスコ","givenNameLang":"ja-Kana"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-11"}],"filename":"fishu_k_66_1.pdf","filesize":[{"value":"4.1 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"url":"https://fra.repo.nii.ac.jp/record/2012111/files/fishu_k_66_1.pdf"},"version_id":"58dafb6d-a326-45ce-bce8-0d366de2215a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Takifugu rubripes; Takifugu niphobles; vasa; germ cell; transplantation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"トラフグおよびクサフグにおけるvasaホモログ遺伝子の単離と発現解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"トラフグおよびクサフグにおけるvasaホモログ遺伝子の単離と発現解析","subitem_title_language":"ja"},{"subitem_title":"Cloning and expression analysis of vasa homolog genes in tiger puffer Takifugu rubripes and grass puffer Takifugu niphobles","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"10","path":["1724312738447"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-11"},"publish_date":"2024-10-11","publish_status":"0","recid":"2012111","relation_version_is_last":true,"title":["トラフグおよびクサフグにおけるvasaホモログ遺伝子の単離と発現解析"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2025-03-31T05:19:55.959983+00:00"}