@article{oai:fra.repo.nii.ac.jp:00000064,
 author = {Masaoka, Tetsuji and 正岡, 哲治 and Nagoya, Hiroyuki and 名古屋, 博之 and Okamoto, Hiroyuki and 岡本, 裕之 and Araki, Kazuo and 荒木, 和男 and Fujiwara, Atsushi and 藤原, 篤志 and Kobayashi, Takanori and 小林, 敬典},
 journal = {DNA鑑定},
 month = {Jul},
 note = {Carp（Cyprinus carpio）, Carassius fish, and goldfish（Carassius auratus）are popular cyprinid species bred for use in aquaculture as a food source, in aquaria, and biological research in many countries. Hybrids derived from a cross between carp and Carassius fish containing the genome of crucian carp and goldfish have been artificially produced for use as food and aquarium fish. We obtained a third generation of diploid and triploid hybrids（F3）derived from crosses between the F2 hybrid of family 1［F1 hybrid（C. carpio ♀ × Carassius cuvieri ♂）♀ × C. carpio ♂］♀ and C. auratus ♂ and cross between the F2 hybrid of family 2［F1 hybrid（C. carpio ♀ × C. cuvieri ♂）♀ × C. cuvieri ♂ ］ ♀ and C. auratus ♂. However, it is difficult to estimate the genome composition of the F3 hybrids by morphological measurement. We previously developed three methods of species distinction between carp and Carassius fish：（1）analysis by PCR amplification using carp- and crucian carp-specific primers targeting the IGF-1 gene region,（2）PCR-RFLP analysis of the TGF-beta gene region, and（3）PCR-RFLP analysis of the mitochondrial 16S ribosomal RNA gene region. In this study, we estimated the genome composition of eight F3 hybrids by these methods. We estimated that five F3 hybrids contained the genomes of C. carpio, C. cuvieri, and C. auratus. Thus,  the five F3 hybrids are considered to be allotriploids and include the genomes of three fish species. We estimated that two F3 hybrids contained the genomes of C. carpio and C. auratus. We also estimated that one F3 hybrid contained the genomes of C. cuvieri and C. auratus . Thus, these three F3 hybrid types are considered to be diploid hybrids. Moreover, all the eight F3 hybrids were estimated to contain the mitochondrial DNA derived from C. carpio as the maternal species.},
 pages = {27--40},
 title = {PCRを用いたコイとフナ類及びキンギョの雑種F3が有するゲノムの推定},
 volume = {12},
 year = {2021},
 yomi = {マサオカ, テツジ and ナゴヤ, ヒロユキ and オカモト, ヒロユキ and アラキ, カズオ and フジワラ, アツシ and コバヤシ, タカノリ}
}