@article{oai:fra.repo.nii.ac.jp:00000085,
 author = {Hongo, Yuki and 本郷, 悠貴 and Kimura, Kei and キムラ, ケイ and Takaki, Yoshihiro and タカキ, ヨシヒロ and Yoshida, Yukari and ヨシダ, ユカリ and Baba, Shuichiro and ババ, シュウイチロウ and Kobayashi, Genta and コバヤシ, ゲンタ and Nagasaki, Keizo and ナガサキ, ケイゾウ and Hano, Takeshi and 羽野, 健志 and Tomaru, Yuji and 外丸, 裕司},
 issue = {1},
 journal = {Scientific Reports},
 month = {Nov},
 note = {Diatoms are one of the most prominent oceanic primary producers and are now recognized to be distributed throughout the world. They maintain their population despite predators, infections, and unfavourable environmental conditions. One of the smallest diatoms, Chaetoceros tenuissimus, can coexist with infectious viruses during blooms. To further understand this relationship, we sequenced the C. tenuissimus strain NIES-3715 genome. A gene fragment of a replication-associated gene from the infectious ssDNA virus (designated endogenous virus-like fragment, EVLF) was found to be integrated into each 41 Mb of haploid assembly. In addition, the EVLF was transcriptionally active and conserved in nine other C. tenuissimus strains from different geographical areas, although the primary structures of their proteins varied. The phylogenetic tree further suggested that the EVLF was acquired by the ancestor of C. tenuissimus. Additionally, retrotransposon genes possessing a reverse transcriptase function were more abundant in C. tenuissimus than in Thalassiosira pseudonana and Phaeodactylum tricornutum. Moreover, a target site duplication, a hallmark for long interspersed nuclear element retrotransposons, flanked the EVLF. Therefore, the EVLF was likely integrated by a retrotransposon during viral infection. The present study provides further insights into the diatom-virus evolutionary relationship.},
 pages = {１--13},
 title = {The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus},
 volume = {11},
 year = {2021},
 yomi = {ホンゴウ, ユウキ and キムラ, ケイ and タカキ, ヨシヒロ and ヨシダ, ユカリ and ババ, シュウイチロウ and コバヤシ, ゲンタ and ナガサキ, ケイゾウ and ハノ, タケシ and トマル, ユウジ}
}