{"created":"2023-06-20T14:02:22.573821+00:00","id":95,"links":{},"metadata":{"_buckets":{"deposit":"2df82ae3-d90d-4a7b-9a31-53b256057860"},"_deposit":{"created_by":10,"id":"95","owners":[10],"pid":{"revision_id":0,"type":"depid","value":"95"},"status":"published"},"_oai":{"id":"oai:fra.repo.nii.ac.jp:00000095","sets":["15"]},"author_link":["503","567"],"control_number":"95","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-02-22","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"e77704","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Metabarcoding and Metagenomics","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Accuracy of PCR amplification is vital for obtaining reliable amplicon-sequencing results by metabarcoding. Here, we performed a comparative analysis of error profiles in the PCR products by 14 different PCR kits using a mock eukaryotic community DNA sample mimicking metabarcoding analysis. To prepare a mock eukaryotic community from the marine environment, equal amounts of plasmid DNA from 40 microalgal species were mixed and used for amplicon-sequencing by a high-throughput sequencing approach. To compare the differences in PCR kits used for this experiment, we focused on the following seven parameters: 1) Quality, 2) Chimera, 3) Blast top hit accuracy, 4) Deletion, 5) Insertion, 6) Base substitution and 7) Amplification bias amongst species. The results showed statistically significant differences (p < 0.05) for all of the seven parameters depending on the PCR kits used. These differences may result from the different DNA polymerases included in each kit, although the result can also be influenced by PCR reaction conditions. Simultaneous analysis of several parameters suggested that kits containing KOD plus Neo (TOYOBO) and HotStart Taq DNA polymerase (BiONEER, CA, US) at the annealing temperature of 65 °C displayed better results in terms of parameters associated with chimeras, top hit similarity and deletions.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Pensoft Publishers","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3897/mbmg.6.77704","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Fraism:10055901"}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Metabarcoing & Metagenomics"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.doi.org/10.3897/mbmg.6.77704","subitem_relation_type_select":"URI"}}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2534-9708","subitem_source_identifier_type":"EISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000494425826","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000494425826"}],"affiliationNames":[{"affiliationName":"Japan Fisheries Research and Education Agency","affiliationNameLang":"en"},{"affiliationName":"水産研究・教育機構","affiliationNameLang":"ja"},{"affiliationName":"スイサンケンキュウキョウイクキコウ","affiliationNameLang":"ja-Kana"}]}],"creatorNames":[{"creatorName":"Nagai, Satoshi","creatorNameLang":"en"},{"creatorName":"長井, 敏","creatorNameLang":"ja"},{"creatorName":"ナガイ, サトシ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Nagai","familyNameLang":"en"},{"familyName":"長井","familyNameLang":"ja"},{"familyName":"ナガイ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Satoshi","givenNameLang":"en"},{"givenName":"敏","givenNameLang":"ja"},{"givenName":"サトシ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"567","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80371962","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80371962"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Tallinn University of Technology","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Sildever, Sirje","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Tokyo Gas Co., Ltd.","affiliationNameLang":"en"},{"affiliationName":"東京ガス株式会社","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Nishi, Noriko","creatorNameLang":"en"},{"creatorName":"ニシ, ノリコ","creatorNameLang":"ja"},{"creatorName":"ニシ, ノリコ","creatorNameLang":"ja-Kana"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Tokyo Gas Co., Ltd.","affiliationNameLang":"en"},{"affiliationName":"東京ガス株式会社","affiliationNameLang":"ja-Kana"}]}],"creatorNames":[{"creatorName":"Tozawa, Satoshi","creatorNameLang":"en"},{"creatorName":"トザワ, サトシ","creatorNameLang":"ja"},{"creatorName":"トザワ, サトシ","creatorNameLang":"ja-Kana"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Tokyo University of Marine Science and Technology","affiliationNameLang":"en"},{"affiliationName":"東京海洋大学","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Basti, Leila","creatorNameLang":"en"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"0000000494425826","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"https://isni.org/isni/0000000494425826"}],"affiliationNames":[{"affiliationName":"水産研究・教育機構","affiliationNameLang":"ja"},{"affiliationName":"Japan Fisheries Research and Education Agency","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"Kobayashi, Takanori","creatorNameLang":"en"},{"creatorName":"小林, 敬典","creatorNameLang":"ja"},{"creatorName":"コバヤシ, タカノリ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"Kobayashi","familyNameLang":"en"},{"familyName":"小林","familyNameLang":"ja"},{"familyName":"コバヤシ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Takanori","givenNameLang":"en"},{"givenName":"敬典","givenNameLang":"ja"},{"givenName":"タカノリ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"503","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70205467","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70205467"}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"Kyushu University","affiliationNameLang":"en"},{"affiliationName":"九州大学","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"Ishino, Yoshizumi","creatorNameLang":"en"},{"creatorName":"イシノ, ヨシズミ","creatorNameLang":"ja"},{"creatorName":"イシノ, ヨシズミ","creatorNameLang":"ja-Kana"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-12-01"}],"displaytype":"detail","filename":"10055901.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","license_note":"CC BY 4.0\n(c)Authors","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"10055901","url":"https://fra.repo.nii.ac.jp/record/95/files/10055901.pdf"},"version_id":"94bd7979-e556-4ba8-ab1b-a5f9c21729ed"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"chimeric sequences","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA polymerase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"high throughput sequencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"metabarcoding","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR-generated artifacts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"18S rRNA gene","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Comparing PCR-generated artifacts of different polymerases for improved accuracy of DNA metabarcoding","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Comparing PCR-generated artifacts of different polymerases for improved accuracy of DNA metabarcoding","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"10","path":["15"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2022-10-03"},"publish_date":"2022-10-03","publish_status":"0","recid":"95","relation_version_is_last":true,"title":["Comparing PCR-generated artifacts of different polymerases for improved accuracy of DNA metabarcoding"],"weko_creator_id":"10","weko_shared_id":-1},"updated":"2024-11-26T00:45:03.229161+00:00"}