WEKO3
アイテム
Immunocytochemical Visualization of Proteins from Cyanobacterial Cells with High Autofluorescence of Phycoerythrin and Phycourobilin
https://fra.repo.nii.ac.jp/records/2001523
https://fra.repo.nii.ac.jp/records/2001523355daa66-19d7-4f24-b400-1a7cb284eb35
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-03-19 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Immunocytochemical Visualization of Proteins from Cyanobacterial Cells with High Autofluorescence of Phycoerythrin and Phycourobilin | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| アクセス権 | ||||||||||||||||
| アクセス権 | open access | |||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||
| 著者 |
増田, 貴子
× 増田, 貴子× Majerová, Dominika
× Piwosz, Kasia
× Tsurumaki, Tatsuhiro
× Fujita, Yuichi
× Prášil, Ondřej
|
|||||||||||||||
| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | Presented is a protocol for visualizing and quantifying a specific protein in cells at the cellular level for the marine cyanobacterium Crocosphaera watsonii, a crucial primary producer and nitrogen fixer in oligotrophic oceans. One of the challenges for marine autotrophic N2 fixers (diazotrophs) is distinguishing probe-derived fluorescence signals from autofluorescence. C. watsonii was selected to represent chlorophyll-, phycoerythrin- and phycourobilin-containing cyanobacteria. The protocol allows for simple and semi-quantitative visualization of proteins in C. watsonii at a single-cell level, enabling investigation of protein production under different environmental conditions to evaluate the metabolic activities of the target cyanobacteria. Furthermore, the fixation and permeabilization methods are optimized to enhance the fluorescence signals from target proteins to distinguish them from autofluorescence, especially from phycoerythrin and phycourobilin. The enhanced signal can be visualized using confocal or widefield fluorescence microscopy. Additionally, fluorescence intensity was semi-quantified using Fiji software. This single-cell analysis workflow allows the evaluation of cell-to-cell variations of specific protein content. The protocol can be performed in any life science laboratory as it requires only standard equipment and can also be easily adapted to other phycoerythrin-containing cyanobacterial cells. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 書誌情報 |
en : JoVE Journal 発行日 2023-09-15 |
|||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | MyJove Corporation | |||||||||||||||
| 言語 | en | |||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||
| 収録物識別子 | 1940-087X | |||||||||||||||
| DOI | ||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | 10.3791/65168 | |||||||||||||||
| 情報源 | ||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||
| 識別子タイプ | Local | |||||||||||||||
| 関連識別子 | 23235016 | |||||||||||||||
| 言語 | ja | |||||||||||||||
| 関連名称 | 水産資源研究所 水産資源研究センター 海洋環境部 | |||||||||||||||
| 関連サイト | ||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||
| 識別子タイプ | URI | |||||||||||||||
| 関連識別子 | https://app.jove.com/t/65168/immunocytochemical-visualization-of-proteins-from-cyanobacterial-cells-with-high-autofluorescence-of-phycoerythrin-and-phycourobilin | |||||||||||||||
| 言語 | en | |||||||||||||||
| 関連名称 | JoVE Journal | |||||||||||||||
| 著者版フラグ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||
