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  1. 学術雑誌論文

Immunocytochemical Visualization of Proteins from Cyanobacterial Cells with High Autofluorescence of Phycoerythrin and Phycourobilin

https://fra.repo.nii.ac.jp/records/2001523
https://fra.repo.nii.ac.jp/records/2001523
355daa66-19d7-4f24-b400-1a7cb284eb35
Item type 学術雑誌論文 / Journal Article(1)
公開日 2024-03-19
タイトル
タイトル Immunocytochemical Visualization of Proteins from Cyanobacterial Cells with High Autofluorescence of Phycoerythrin and Phycourobilin
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
著者 増田, 貴子

× 増田, 貴子

WEKO 1174
ORCID 0000-0002-1970-8645

en Masuda, Takako

ja 増田, 貴子


ja-Kana マスダ, タカコ

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Majerová, Dominika

× Majerová, Dominika

en Majerová, Dominika
The Czech Academy of Sciences

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Piwosz, Kasia

× Piwosz, Kasia

en Piwosz, Kasia
The Czech Academy of Sciences
National Marine Fisheries Research Institute

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Tsurumaki, Tatsuhiro

× Tsurumaki, Tatsuhiro

en Tsurumaki, Tatsuhiro
The Czech Academy of Sciences

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Fujita, Yuichi

× Fujita, Yuichi

en Fujita, Yuichi
Nagoya University

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Prášil, Ondřej

× Prášil, Ondřej

en Prášil, Ondřej
The Czech Academy of Sciences

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抄録
内容記述タイプ Abstract
内容記述 Presented is a protocol for visualizing and quantifying a specific protein in cells at the cellular level for the marine cyanobacterium Crocosphaera watsonii, a crucial primary producer and nitrogen fixer in oligotrophic oceans. One of the challenges for marine autotrophic N2 fixers (diazotrophs) is distinguishing probe-derived fluorescence signals from autofluorescence. C. watsonii was selected to represent chlorophyll-, phycoerythrin- and phycourobilin-containing cyanobacteria. The protocol allows for simple and semi-quantitative visualization of proteins in C. watsonii at a single-cell level, enabling investigation of protein production under different environmental conditions to evaluate the metabolic activities of the target cyanobacteria. Furthermore, the fixation and permeabilization methods are optimized to enhance the fluorescence signals from target proteins to distinguish them from autofluorescence, especially from phycoerythrin and phycourobilin. The enhanced signal can be visualized using confocal or widefield fluorescence microscopy. Additionally, fluorescence intensity was semi-quantified using Fiji software. This single-cell analysis workflow allows the evaluation of cell-to-cell variations of specific protein content. The protocol can be performed in any life science laboratory as it requires only standard equipment and can also be easily adapted to other phycoerythrin-containing cyanobacterial cells.
言語 en
書誌情報 en : JoVE Journal

発行日 2023-09-15
出版者
出版者 MyJove Corporation
言語 en
ISSN
収録物識別子タイプ EISSN
収録物識別子 1940-087X
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.3791/65168
情報源
関連タイプ isIdenticalTo
識別子タイプ Local
関連識別子 23235016
言語 ja
関連名称 水産資源研究所 水産資源研究センター 海洋環境部
関連サイト
関連タイプ isIdenticalTo
識別子タイプ URI
関連識別子 https://app.jove.com/t/65168/immunocytochemical-visualization-of-proteins-from-cyanobacterial-cells-with-high-autofluorescence-of-phycoerythrin-and-phycourobilin
言語 en
関連名称 JoVE Journal
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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