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Characterization of eight types of 17β-hydroxysteroid dehydrogenases from the Japanese sardine Sardinops melanostictus: The probable role of type 12a in ovarian estradiol synthesis
https://fra.repo.nii.ac.jp/records/2002161
https://fra.repo.nii.ac.jp/records/20021612a6269f1-3421-4ccf-8cd6-49f288ffea3a
| 名前 / ファイル | ライセンス | アクション |
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Nyuji et al Characterization of eight types figs.pdf (1.5 MB)
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Nyuji et al Characterization of eight types.pdf (355 KB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2024-04-19 | |||||
| タイトル | ||||||
| タイトル | Characterization of eight types of 17β-hydroxysteroid dehydrogenases from the Japanese sardine Sardinops melanostictus: The probable role of type 12a in ovarian estradiol synthesis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Hsd17b; Estradiol-17; Steroidogenesis; Ovary; Teleost | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
入路, 光雄
× 入路, 光雄× 本郷, 悠貴× 風藤, 行紀× 米田, 道夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 17β-hydroxysteroid dehydrogenases (Hsd17bs) play a critical role in sex steroid biosynthesis. Although multiple types of Hsd17b have been found in fish, there is limited research on their expression and function. Recently, we succeeded in identifying eight types of Hsd17b (types 3, 4, 7, 8, 10, 12a, 12b, and 14) by RNA sequencing in the Japanese sardine Sardinops melanostictus, a commercially important clupeoid fish; however, a homologous sequence of Hsd17b1, which catalyzes the key reaction of estradiol-17β (E2) synthesis, was absent. Here, we aimed to identify the Hsd17b type that plays a major role in E2 synthesis during ovarian development in Japanese sardine. The cDNAs encoding those eight types of Hsd17b were cloned and sequenced. The expressions of hsd17b3, hsd17b12a, and hsd17b12b were higher in ovary than in testis. In particular, hsd17b12a was predominantly expressed in the ovary. Expression of hsd17b3, hsd17b4, hsd17b12a, and hsd17b12b in the ovary increased during ovarian development. The enzymatic activities of Hsd17b3, Hsd17b12a, and Hsd17b12b were evaluated by expressing their recombinants in human embryonic kidney 293T cells. Hsd17b12a and Hsd17b12b catalyzed the conversion of androstenedione (AD) to testosterone (T) and estrone (E1) to E2. The results of in vitro bioassays using sardine ovaries indicated that E2 is synthesized from pregnenolone via AD and T, but not E1. These results suggest that Hsd17b12a plays a major role in E2 synthesis in sardine ovary by catalyzing the conversion of AD to T. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : General and Comparative Endocrinology 巻 347, p. 114423, 発行日 2024-02-01 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0016-6480 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.ygcen.2023.114423 | |||||
| 情報源 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | Local | |||||
| 関連識別子 | 23331002 | |||||
| 言語 | ja | |||||
| 関連名称 | 水産技術研究所 養殖部門 まぐろ養殖部 （長崎） | |||||
| 関連サイト | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S0016648023002289 | |||||
| 言語 | en | |||||
| 関連名称 | Elsevier : General and Comparative Endocrinology | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
