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  1. 学術雑誌論文

Characterization of eight types of 17β-hydroxysteroid dehydrogenases from the Japanese sardine Sardinops melanostictus: The probable role of type 12a in ovarian estradiol synthesis

https://fra.repo.nii.ac.jp/records/2002161
https://fra.repo.nii.ac.jp/records/2002161
2a6269f1-3421-4ccf-8cd6-49f288ffea3a
名前 / ファイル ライセンス アクション
Nyuji Nyuji et al Characterization of eight types figs.pdf (1.5 MB)
Nyuji Nyuji et al Characterization of eight types.pdf (355 KB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2024-04-19
タイトル
タイトル Characterization of eight types of 17β-hydroxysteroid dehydrogenases from the Japanese sardine Sardinops melanostictus: The probable role of type 12a in ovarian estradiol synthesis
言語 en
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 Hsd17b; Estradiol-17; Steroidogenesis; Ovary; Teleost
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 入路, 光雄

× 入路, 光雄

WEKO 1178
e-Rad 50732426
ORCID 0000-0003-1436-3016

en Nyuji, Mitsuo

ja 入路, 光雄


ja-Kana ニュウジ, ミツオ

Search repository
本郷, 悠貴

× 本郷, 悠貴

WEKO 902
e-Rad 20737316
ORCID 0000-0003-1434-1220

en Hongo, Yuki

ja 本郷, 悠貴


ja-Kana ホンゴウ, ユウキ

Search repository
風藤, 行紀

× 風藤, 行紀

WEKO 775
e-Rad 60399996

en Kazeto, Yukinori

ja 風藤, 行紀


ja-Kana カゼトウ, ユキノリ

Search repository
米田, 道夫

× 米田, 道夫

WEKO 257
e-Rad 30450787

en Yoneda, Michio

ja 米田, 道夫


ja-Kana ヨネダ, ミチオ

Search repository
抄録
内容記述タイプ Abstract
内容記述 17β-hydroxysteroid dehydrogenases (Hsd17bs) play a critical role in sex steroid biosynthesis. Although multiple types of Hsd17b have been found in fish, there is limited research on their expression and function. Recently, we succeeded in identifying eight types of Hsd17b (types 3, 4, 7, 8, 10, 12a, 12b, and 14) by RNA sequencing in the Japanese sardine Sardinops melanostictus, a commercially important clupeoid fish; however, a homologous sequence of Hsd17b1, which catalyzes the key reaction of estradiol-17β (E2) synthesis, was absent. Here, we aimed to identify the Hsd17b type that plays a major role in E2 synthesis during ovarian development in Japanese sardine. The cDNAs encoding those eight types of Hsd17b were cloned and sequenced. The expressions of hsd17b3, hsd17b12a, and hsd17b12b were higher in ovary than in testis. In particular, hsd17b12a was predominantly expressed in the ovary. Expression of hsd17b3, hsd17b4, hsd17b12a, and hsd17b12b in the ovary increased during ovarian development. The enzymatic activities of Hsd17b3, Hsd17b12a, and Hsd17b12b were evaluated by expressing their recombinants in human embryonic kidney 293T cells. Hsd17b12a and Hsd17b12b catalyzed the conversion of androstenedione (AD) to testosterone (T) and estrone (E1) to E2. The results of in vitro bioassays using sardine ovaries indicated that E2 is synthesized from pregnenolone via AD and T, but not E1. These results suggest that Hsd17b12a plays a major role in E2 synthesis in sardine ovary by catalyzing the conversion of AD to T.
言語 en
書誌情報 en : General and Comparative Endocrinology

巻 347, p. 114423, 発行日 2024-02-01
出版者
出版者 Elsevier
言語 en
ISSN
収録物識別子タイプ PISSN
収録物識別子 0016-6480
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.1016/j.ygcen.2023.114423
情報源
関連タイプ isIdenticalTo
識別子タイプ Local
関連識別子 23331002
言語 ja
関連名称 水産技術研究所 養殖部門 まぐろ養殖部 (長崎)
関連サイト
関連タイプ isIdenticalTo
識別子タイプ URI
関連識別子 https://www.sciencedirect.com/science/article/pii/S0016648023002289
言語 en
関連名称 Elsevier : General and Comparative Endocrinology
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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