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産業排水の魚類に及ぼす影響に関する研究（第一報）血清蛋白の濾紙電気泳動像に依る影響の判定について
https://fra.repo.nii.ac.jp/records/2005084
https://fra.repo.nii.ac.jp/records/20050849a72afa3-b36f-40b8-accf-3d3d9baab9eb
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-05-17 | |||||
| タイトル | ||||||
| タイトル | 産業排水の魚類に及ぼす影響に関する研究（第一報）血清蛋白の濾紙電気泳動像に依る影響の判定について | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Study on the effects of industrial waste and chemicals upon the fish. (On the determination of their effects by the paper electrophoretic method of serum) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
藤谷, 超
× 藤谷, 超× 千国, 史郎 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Though the studeies of this field had been reported by many people, most of them were concerned with the lethal dose or minimum active dose for fish. They had discussed the ecological factor more in detail, but there were few treatise on physiological one. Physiological approach would be necessary for the investigation of their effects upon the aquatic animals. We attempted to take the paper electrophoretic method of serum as the one of physiological method in this field. In recent years, paper electrophoretic method had developed in the medical science, especially in clinical medicine, and was also applied to the physiological chemistry of fisheries investigation. The present method was almost the same as the one usually taken in the medical study, but some parts of them were modified suitably for our case. The veronal buffer solution(pH=8.6 μ=0.05) and B. P. B. acetic acid solution were used on the electrolysis solution and the dyeing solution, and the serum was made of the blood collected from ampitaled anal part of a fish. As the blood, left alone on a glass dish, was divided into two parts, the serum was centrifuged to reseparate from the clot completely. A pipette of the serum (0.02 ml. ) was put on the paper and electric current of 0.5 mA./cm. width of the paper was given for 5 hrs. The electrophoretic patterns on the paper thus taken and the curves representing them were shown in figure. From them we could say : 1) The concentrations (dosages) of acid and alkaline which had affected upon the paper electrophoretic patterns and hurt of the gill tissues were almost equal. 2) The pattern for suffocated fish differed from the one for normal fish. 3) We could find some differences between the patterns for normal fish and for fish in malnutrition. 4) The fish affected by pulp mill waste could be discriminated from the normal one experimentally and also there was a notable difference between the pattern for normal fish and that obtained for the fish sustained in the sea for 12 hrs. under the influence of waste from pulp mill factory. 5) By this method, the physiological effective dose of pulp mill waste for fish was estimated to be a concentration of as low. as 30-50 mg/L in C. O. D. value. 6) Therefore in our estimations, the effect of the wastes upon the fish should not be ignored, even if their concentration in sea water might be as low as the one which had not been noticed practically. | |||||
| 言語 | en | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 1) 血清蛋白の沪紙電気泳動像に依って,魚類に及ぼす産業廃水の影響を検討する方法を実験,考察した。2) 酸,アルカリなどの影響も,この方法で示し得るが,この場合泳動像に影響が現われる濃度は,鰓の組織に損傷が生ずる濃度と略一致する所から,泳動像に現われる影響は,鰓の損傷に起因するものと考える。3) 窒息の状態の魚の泳動像にも異状が見られるが,窒息死したものの中には泳動像に余り変化のないものもあった。これは酸素欠乏の度合及び死に致る迄の時間の差異などに起因するものと思われるが判然としたことは知り得なかった。4)、栄養不良と認められる自然死の魚の泳動像は,その異状が極めて顕著である。5) パルブ工場廃水の波及する水域に置かれた魚類の影響はその泳動像から明確に判定することが出来その置かれた条件の程度をも併せて知ることが出来る。6) 泳動像の変化から求められる,パルプ工場廃水の魚類生理に及ぼす影響は可成り低濃度のものであり,C.O.D.で30~50ppmの濃度ではすでに影響が認められる。7) パルブ廃水の影響を受けた魚体の泳動像は,窒息死の場合の像に類似しており,パルブ廃水の魚類に及ぼす影響は,魚体に呼吸困難の状態を与えている疑いが濃厚である。 | |||||
| 言語 | ja | |||||
| bibliographic_information |
ja : 内海区水産研究所研究報告 en : Bulletin of Naikai Regional Fisheries Research Laboratory 巻 11, p. 41-52, ページ数 12, 発行日 1958-05 |
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| 出版者 | ||||||
| 出版者 | 内海区水産研究所 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Naikai Regional Fisheries Research Laboratory | |||||
| 言語 | en | |||||
| item_10002_source_id_9 | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0497-5022 | |||||
| item_10002_source_id_11 | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00176718 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | nai_k_1105 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010841464 | |||||
| 言語 | ja | |||||
| 関連名称 | 日本農学文献記事索引(agriknowledge) | |||||
