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Re-evaluation of nuclear mitochondrial pseudogenes (NUMTs) and heteroplasmy in the Japanese spiny lobster Panulirus japonicus
https://fra.repo.nii.ac.jp/records/2008600
https://fra.repo.nii.ac.jp/records/2008600b72b5719-797a-482a-b894-148e5b840e23
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-06-24 | |||||
| タイトル | ||||||
| タイトル | Re-evaluation of nuclear mitochondrial pseudogenes (NUMTs) and heteroplasmy in the Japanese spiny lobster Panulirus japonicus | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Japanese spiny lobster; NUMTs; mitochondrial DNA; heteroplasmy; sample degradation; cDNA | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
張, 成年
× 張, 成年× 安池, 元重× 柳本, 卓 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The Japanese spiny lobster Panulirus japonicus has been reported to harbor a numbers of nuclear mitochondrial pseudogenes (NUMTs) and heteroplasmy. However, distinguishing phylogenetically young NUMTs, heteroplasmy, and PCR-cloning artefacts may be challenging. In addition, greater degradation for mtDNA than nuclear DNA in elderly tissue specimens may promote amplification of NUMTs. In this study, we performed clone library-based nucleotide sequence analysis of the partial mtDNA COI gene using genomic DNA and cDNA obtained from fresh tissues of the Japanese spiny lobster and genomic DNA obtained from three crustacean and three fish species. Minor nucleotide substitutions between clones in an individual were ubiquitously observed in all species examined including the lobster cDNA, suggesting that most of these were artefacts. Rarely, a few clones were most likely to have originated from heteroplasmic copies, as they had skewed nucleotide substitutions at the third codon. The Japanese spiny lobster is more likely than others to detect NUMTs, while the detection of NUMTs may be somewhat suppressed using genomic DNA obtained from fresh tissue. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Crustacean Research 巻 53, p. 27-36, ページ数 10, 発行日 2024-04-12 |
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| 出版者 | ||||||
| 出版者 | Carcinological Society of Japan | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | 日本甲殻類学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2189-5317 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0287-3478 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.18353/crustacea.53.0_27 | |||||
| 情報源 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | Local | |||||
| 関連識別子 | 23342125 | |||||
| 言語 | ja | |||||
| 関連名称 | 水産技術研究所 環境・応用部門 沿岸生態システム部 （横浜） | |||||
| 関連サイト | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/crustacea/53/0/53_27/_article/-char/en | |||||
| 言語 | en | |||||
| 関連名称 | J-STAGE | |||||
| 著者版フラグ | ||||||
| 出版タイプ | SMUR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_71e4c1898caa6e32 | |||||
