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アコヤガイとハボウキガイの外とう膜における粘液物質の行動について
https://fra.repo.nii.ac.jp/records/2009001
https://fra.repo.nii.ac.jp/records/200900171440ff3-12af-4b53-b449-f65a2a724296
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-06-27 | |||||
| タイトル | ||||||
| タイトル | アコヤガイとハボウキガイの外とう膜における粘液物質の行動について | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Behavior of the mucus substance in the mantle of Pinctada martensiiand Pinna attenuata (Bivalves). | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
中原, 晧
× 中原, 晧 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | 1) アコヤガイでは外とう膜殻側面（外面）の粘液細胞は特に中心域に多く，ハボウキガイでは前面にほぼ一様に分布している。 2) 粘液細胞の果粒は分泌されて液状となり，外とう膜と貝殻の間に拡がる。アコヤガイでは中心域で多量に分泌された粘液は中心域から周辺部に向かって流れる。 3) 粘液物質は貝殻真珠層の有機質を形成するものと思われる。 |
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| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | (1) Bevelander and Benzer (1948) stated that the mucous substance of the mollusc mantle was associated with shell mineralization. During the past few years, it has been further suggested (Wilbur 1960, Kado 1960, Tsujii 1960) that the mucous substance plays a significant role in shell formation. From biochemical evidence, Horiguchi (1956) stated that the mantle mucilage contains a large amount of sulfomucopolysaccharide. Tanaka and Hatano (1955), and Kado (1960) discussed the specific affinity of mucous substance of mollusc mantle for calcium. Wada (1961) reported that the organic matrix, upon which crystal formation occurs, to be metachromatic. Beedham (1958) suggested that the mucous secreted between the mantle and shell acts as a lubricant for movement of the mantle. The author reported (Nakahara 1961a) that the mucous substance is incorporated directly into the calcified nacreous layer. Further, with the method of tetracycline administration (Nakahara 1961b), it was demonstrated that the nacreous layer in the dorsal part of the shell of Pinctada grows more rapidly than the nacre of the ventral or marginal part. This fact reveals that the whole area, including the dorsal part of the mantle is important in shell formation. Former investigations of the mantle refer for the most part to the marginal or membranous area. We have shown, however, the need to observe whole area of the mantle including the part which covers the visceral mass. (2) Japanese pearl oysters (Pinctada martensii) used for the study, were 3-4 years old, and kept at a pearl farm in Ago-bay. Pinna attenuata were collected in Ago-bay during the summer. Materials were carefully fixed in 1% OsO4 solution buffered at pH 7.4, Regaud's solution, and absolute alcohol. Methacrylate embedding, commonly used in electron microscopy, was followed after osmic acid fixation, and the paraffin method for the others. Sections were stained with 1/10,000 toluidine blue aqueous solution. Haematoxylin-eosin stain and some other histological staining and histochemical techniques were also used. (3) The mucous cells develop over the entire mantle surface of Pinctada (Ojima 1950, 1952). On the shell surface, development of the mucous cells show a marked difference between the central and pallial zone. Well developed masses of the mucous cells were located in the central zone of the mantle, in contrast to the pallial and marginal zone where relatively few of these cells occur (Figs. 1-4). Two kinds of granules are distinguished in the mucous cells of the central area. One of them is well stained with eosin, slightly with toluidine blue. The other is not stained with eosin, but is well stained with toluidine blue and haematoxylin. Both of them show metachromasia with toluidine blue, and are positive to the PAS reaction. Hayashi (1954) described these cells and assumed that the former is albuminous, and the latter is a calcareous gland. In the present study, with the aid of microincineration, calcium was observed to be present in almost the same amount in both cells. In Pinna attenuata, mucous cells are evenly distributed on the shell surface of the mantle (Figs. 5-7). Most of the mucous granules in the mucous cells are well stained with toluidine blue and show intense metachromasia. (4) Mucous granules are secreted into the space between the mantle and shell. Just after the secretion, they become fluid and spread over the epithelial surface (Figs. 2, 3, 5, 6). In Pinctada observations following carmine injection by the author (Nakahara 1962), show the mucous extends from the central zone to the marginal zone of mantle. Secreted mucous substance shows metachromasia, and the matrix of the nacre decalcified with EDTA also shows intense metachromasia. The mucous layer shows a fine lamellar structure, and contains very small particles. In Pinna mantle fixed in alcohol (Fig. 7), the mucous substance forms a fibrous or membranous structure, and shows marked birefringence (mucous layer fixed in osmic acid showing no birefringence). This transformation may be caused by the dehydration and coagulation with alcohol. During the present study, the author observed with the method of radioautography, that the Ca45 injected in the adductor muscle of Pinctada transferred into the mucous of the mantle within 30 minutes. This phenomenon further supports the contention that the calcium is combined with mucous substance in the course of shell formation. (5) On the basis of our present observations, we have shown that mantle mucous is incorporated, and becomes an integral part of the calcified nacre. The role of the mucous reported here is similar to that previously described in connection with pearl formation by the author (Nakahara 1961a). |
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| 言語 | en | |||||
| 書誌情報 |
ja : 国立真珠研究所報告 en : Bulletin of the National Pearl Research Laboratory 巻 8, p. 871-878, ページ数 8, 発行日 1962-09-30 |
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| 出版者 | ||||||
| 出版者 | 国立真珠研究所 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | National Pearl Research Laboratory | |||||
| 言語 | en | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00091717 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | pearl_k_871 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://jp-pearl.com/wp-content/uploads/2018/04/houkoku008.pdf#001 | |||||
| 言語 | ja | |||||
| 関連名称 | 日本真珠振興会Archive | |||||
