| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-07-17 |
| タイトル |
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タイトル |
Assessing the transmission risk of red sea bream iridovirus (RSIV) in environmental water: insights from fish farms and experimental settings |
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言語 |
en |
| 言語 |
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言語 |
eng |
| キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
IDW; Megalocytivirus; Pagrus major; biosecurity; eDNA; environmental DNA; iron flocculation; waterborne virus. |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
河東, 康彦
髙田, 優三
水野, かおり
| en |
Mizuno, Kaori
Ehime Fisheries Research Center
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| ja |
水野, かおり
愛媛県農林水産研究所水産研究センター
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| ja-Kana |
ミズノ, カオリ
|
Search repository
原川, 翔伍
| en |
Harakawa, Shogo
Ehime Fisheries Research Center
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| ja |
原川, 翔伍
愛媛県農林水産研究所水産研究センター
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| ja-Kana |
ハラカワ, ショウゴ
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Search repository
吉原, 勇作
| en |
Yoshihara, Yusaku
Ainan Town Fisheries Division
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| ja |
吉原, 勇作
愛南町水産課
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| ja-Kana |
ヨシハラ, ユウサク
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Search repository
中川, 徹優
黒部, 智史
川上, 秀昌
| en |
Kawakami, Hidemasa
Ehime Fisheries Research Center
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| ja |
川上, 秀昌
愛媛県農林水産研究所水産研究センター
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| ja-Kana |
カワカミ, ヒデマサ
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Search repository
伊東, 尚史
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Aquatic animal viruses are considered to be transmitted via environmental water between fish farms. This study aimed to understand the actual transmission risk of red sea bream iridovirus (RSIV) through environmental water among fish farms. An environmental DNA (eDNA) method using iron-based flocculation coupled with large-pore filtration was used to monitor RSIV DNA copies in seawater from fish farms and from an experimental infection model. RSIV dispersion in seawater from a net pen where the disease outbreak occurred was visualized by the inverse distance weighting method using multiple-sampling data sets from a fish farm. The analysis demonstrated that the center of the net pen had a high viral load, and RSIV seemed to be quickly diluted by the tidal current. To evaluate the transmission risk of RSIV in environmental water, the red sea bream Pagrus major (approximately 10 g) was exposed to RSIV-contained seawater (103, 104, 105, 106, and 107 copies/L) for 3 days, which mimicked field exposure. A probit analysis of the challenge test indicated that the inferred infection rates of seawater containing 105.9 copies/L and 103.1 copies/L of RSIV were 50% and 0.0001%, respectively. In the surveillance for 3 years at 10 fixed points (n = 306), there were only seven samples in which the viral load exceeded 104 copies/L in seawater. These results suggest that the transmission of RSIV among fish farms via seawater is highly associated with the distance between the net pens, and the environmental water is not always an infection source for the transmission of RSIV between fish farms. IMPORTANCE Our surveillance of viral loads for red sea bream iridovirus (RSIV) by monitoring environmental DNA in fish farms suggested that the viral loads in the seawater were low, except for the net pens where RSIV outbreaks occurred. Furthermore, our experimental infection model indicated that the infection risk of RSIV-contained seawater with less than 103 copies/L was extremely low. The limited risk of environmental water for transmission of RSIV gives an insight that RSIV could be partly transmitted between fish farms due to the movement of equipment and/or humans from the fish farm where the disease outbreaks. Since our data suggest that seawater can function as a potential wall to reduce the transmission of RSIV, biosecurity management, such as disinfection of equipment associated with fish farms could be effective, even in the semi-open system aquaculture that the environmental water can be freely transferred, to reduce the risk of RSIV outbreaks. |
|
言語 |
en |
| 書誌情報 |
en : Microbiology Spectrum
巻 11,
号 5,
p. e0156723,
発行日 2023-09-22
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| 出版者 |
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出版者 |
American Society for Microbiology |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2165-0497 |
| PubMed番号 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
37737592 |
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言語 |
en |
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関連名称 |
Pub Med |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1128/spectrum.01567-23 |
| 情報源 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
Local |
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関連識別子 |
23336075 |
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言語 |
ja |
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関連名称 |
水産技術研究所 養殖部門 病理部 |
| 関連サイト |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
URI |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/37737592/ |
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言語 |
en |
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関連名称 |
Pub Med |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
kawato-et-al-2023-assessing-the-transmission-risk-of-red-sea-bream-iridovirus-(rsiv)-in-environmental-water-insights (1).pdf (2.2 MB)
