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遺伝資源としてのマガキCrassostrea gigasの特性評価と保存に関する研究
https://fra.repo.nii.ac.jp/records/2010781
https://fra.repo.nii.ac.jp/records/2010781844d49ac-5fa3-48b5-8839-5d7781da5350
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fra_k_4_40.pdf (8.0 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2024-10-02 | |||||
| タイトル | ||||||
| タイトル | 遺伝資源としてのマガキCrassostrea gigasの特性評価と保存に関する研究 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Evaluation of characteristics and preservation of Pacific oyster, Crassostrea gigas, in view of the genetic resources | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Pacific oyster; Crassostrea gigas; Genetics; Heritability; Cryopreservation | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
薄, 浩則
× 薄, 浩則 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Differences in characteristics among local populations of Pacific oyster (Crassostrea gigas) in Japan, evaluation of several characteristics in their genetics and environments, estimation of heritability for improvement of economic traits, and preservation of sperm and larvae were studied on the basis of genetic resources. Growth, mortality and other characteristics of local populations (Hiroshima, Miyagi, Ariake and Goto) were evaluated in parental and progeny groups cultured in Hiroshima Bay. For both generations, the Miyagi population grew fastest and the Ariake and Goto populations grew slower. Though the mortality was not significantly different for progeny cultured in pocket mesh bags, the progeny from Goto showed significantly higher mortality among progenies cultured by the collector method. Progeny from Ariake showed significantly lower mortality among the other progenies when cultured in narrow (10 mm) opening mesh bags and is thought to be the most tolerant to deterioration of living environment. Progeny of the Ariake population also had a spiny projection on their valves and showed a higher biomass rate (volume after narcotization/volume before narcotization). Heritabilities for growth and survival of larvae and adult C. gigas were estimated by nested design (3 male ×5 female). The heritability for survival rate (h2s+d = 0.85) between May and October of one-year-old oysters was larger than that for whole weight in October (h2s+d ≦ 0.4). Selection for improvement of survival was thought to be more effective than growth. No significant correlations in growth and survival between larvae and adults suggested that selection of larvae must be ineffective. To estimate the influence of differences of spat locality and of culturing spot to characters of oyster, spats of Miyagi and Hiroshima populations were cultured simultaneously at four spots in Hiroshima Bay and three-way ANOVA were performed. Though spat locality and culturing spot both significantly influenced many characters, shell width/shell length, density of shell, water content of flesh and water content of shell were not influenced by spat locality. Color of the mantle edge and internal shell cavity rate were influenced by spat locality more intensely than culturing spot, then, shell width/shell length and water content of flesh were influenced by spot more intensely than locality. Intensities of influence of interactions between locality and spot were obviously different among characters of oysters. RFLP analysis of calmodulin intron3 for oysters collected at shores in Ariake Bay aroused the suspicion of natural hybridization between spermatozoa of C. gigas and ova of C. sikamea. Artificial crossbreeding between C. gigas and C. sikamea discriminated by RFLP of 16SrRNA gene revealed that the same type of calmodulin intron3 as oysters collected in Ariake bay could yield in spats. Moreover, 0.6~5.7% of ova of C. gigas inseminated by spermatozoa of C. sikamea had developed and a few spats had set. To determine an effective method for the cryopreservation of C. gigas sperm and larvae, diluents containing cryoprotectants, cooling methods and developmental stages were tested. For the sperm, the combination of a diluent containing 8% (v/v) dimethyl sulfoxide (DMSO), 50mM sucrose and 6mM reduced glutathione with cooling in liquid nitrogen vapor showed the highest relative larval yield (45.9%). Fertility with sperm cryopreserved at a concentration of 105 spermatozoa/200 u L was equivalent to that with fresh sperm when ova were inseminated in a concentration of 103 spermatozoa per ovum. Although spermatozoa cryopreserved for 4 years showed lower viability (40.3~53.3%) than the short-term cryopreserved spermatozoa (60~70%), the ratio of normal D-shaped larvae and survival rate at 6 days after fertilization were 78.0 and 77.4%, respectively. For the larvae, using 1/4 concentration seawater containing 1.0M DMSO and 250mM trehalose as the cryopreservative suspension, and cooling to -35℃ at -1℃/min and kept at this temperature for 5 min before plunging into liquid nitrogen, a oyster spat could yield from cryopreserved trochophore larvae (just before making shell gland) after 8 months of cyopreservation. This indicates that it is possible to establish cryopreservation of the Pacific oyster larvae by further improving the technique. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 水産総合研究センター研究報告 en : Bulletin of Fisheries Research Agency 巻 4, p. 40-104, ページ数 65, 発行日 2002-09 |
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| 出版者 | ||||||
| 出版者 | 水産総合研究センター | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1346-9894 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11589591 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | fra_k_4_40 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010662532 | |||||
| 関連名称 | 日本農学文献記事索引（AgriKnowledge） | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
