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Eukaryotes from the hepatopancreas of lobster phyllosoma larvae
https://fra.repo.nii.ac.jp/records/2010848
https://fra.repo.nii.ac.jp/records/201084890b11fef-0e97-4e1a-91bd-76173fd6051b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fra_k_20_1.pdf (716.5 KB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2024-10-02 | |||||||
| タイトル | ||||||||
| タイトル | Eukaryotes from the hepatopancreas of lobster phyllosoma larvae | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | prey organisms; phyllosoma larvae; Panulirus; scyllarid; 18SrDNA | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
鈴木, 伸明
× 鈴木, 伸明× 村上, 恵祐× TAKEYAMA, Haruko
× 張, 成年 |
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | In order to investigate unknown prey organisms for lobster phyllosoma larvae, molecular approach was developed and adopted using the laboratory-reared and wild phyllosoma samples. The central domain of 18S rDNA was amplified via nested PCR using crude DNAs extracted from the hepatopancrea of the larvae and cloned into plasmid. Clones having 18S rDNA different from the hosts were screened by RFLP analysis, and the nucleotide sequences determined were subjected to FASTA homology search and phylogenetic analysis to identify the source of eukaryotic organisms. Feasibility of this method was confirmed in the laboratory-reared larvae of the Japanese spiny lobster (Panulirus japonicus) fed exclusively on either gonad of common mussel (Mytilus edulis) or Artemia nauplii. From the two wild-caught larvae (Scyllarus sp. and P. japonicus), 178 and 168 clones were isolated, of which 132 and 4 clones had different restriction profiles from the hosts, respectively. DNAs of Cnidaria and Urochordata were observed from the scyllarid larva, while those of fungi and host variant were observed in P. japonicus larva. | |||||||
| 言語 | en | |||||||
| 書誌情報 |
ja : 水産総合研究センター研究報告 en : Bulletin of Fisheries Research Agency 巻 20, p. 1-7, ページ数 7, 発行日 2007-02 |
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| 出版者 | ||||||||
| 出版者 | 水産総合研究センター | |||||||
| 言語 | ja | |||||||
| ISSN | ||||||||
| 収録物識別子タイプ | PISSN | |||||||
| 収録物識別子 | 1346-9894 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA11589591 | |||||||
| 情報源 | ||||||||
| 識別子タイプ | Local | |||||||
| 関連識別子 | fra_k_20_1 | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
