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魚類細胞におけるストレスによって誘発されるオートファジーの観察
https://fra.repo.nii.ac.jp/records/2010869
https://fra.repo.nii.ac.jp/records/20108694b647e4f-d2ed-4b10-9d54-0ceac52db010
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fra_k_26_23.pdf (774.2 KB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-10-02 | |||||
| タイトル | ||||||
| タイトル | 魚類細胞におけるストレスによって誘発されるオートファジーの観察 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Observation of Stress-Induced Autophagy in Fish Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | autophagy; protein degradation; amino acids starvation; heat stress; microtubuleassociated protein 1-light chain 3 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| その他（別言語等）のタイトル | ||||||
| その他のタイトル | 魚類細胞におけるストレスによって誘発されるオートファジーの観察 | |||||
| 言語 | ja | |||||
| 著者 |
藪, 健史
× 藪, 健史× 山下, 倫明 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Starvation is one of important stress conditions that cause various physiological dysfunctions and deteriorate fish quality as a food. Here, we developed a biomarker to detect the state of stress and/or starvation in fish and cultured cells. In particular, we focused on the autophagic pathway that forms autophagic vacuoles, i.e . autophagosomes, in the starved cells by the withdrawal of amino acids from culture medium. The autophagy has been characterized to be an apoptotic pathway induced by stress conditions, such as starvation, heat shock and hypoxia, followed by intracellular protein degradation （bulk degradation） differs from the ubiquitin-proteasome system. We used fish cultured cells as a model to evaluate the influence and the degree of biochemical changes by heat stress and amino acids starvation. A microtubule binding protein that localized in autophagosome membranes can be used as a molecular marker for induction of autophagy. We established stable transformants of ZE cell line introduced with a fusion protein of green fluorescent protein （GFP） and microtubule-associated protein 1-light chain 3 （MAP1- LC3） to detect fluorescent autophagosome associated with the GFP-MAP1-LC3-fusion protein. The induction of autophagy was examined under amino acids starvation and heat stress conditions. The high temperature and amino acids withdrawal in the ZE cells induced autophagy in time-dependent manner by counting the number of fluorescent particles localized with GFP-LC3 fusion protein in the cell. The autophagy induced by the amino acids withdrawal was suppressed in the presence of phosphatidylinositol 3 kinase inhibtor, 3-methyladenine, in the culture medium and by overexperssion of the bcl-2 gene. A phosphatidylinositol 3 kinase, target of rapamycin （TOR）, mediated autophagic signaling pathway that executes the starvation induced-autophagy. Therefore, we proposed a new biological model of autophagic signaling pathway induced under heat stress and amino acid starvation conditions in fish cultured cells. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 水産総合研究センター研究報告 en : Bulletin of Fisheries Research Agency 巻 26, p. 23-28, ページ数 6, 発行日 2008-12 |
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| 出版者 | ||||||
| 出版者 | 水産総合研究センター | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1346-9894 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11589591 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | fra_k_26_23 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010770087 | |||||
| 関連名称 | 日本農学文献記事索引（AgriKnowledge） | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
