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魚類の胚発生におけるオートファジーが関与する卵黄吸収機構
https://fra.repo.nii.ac.jp/records/2010870
https://fra.repo.nii.ac.jp/records/201087062c5d040-7dfe-42e0-a383-5b322c151f5f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fra_k_26_29.pdf (839.5 KB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-10-02 | |||||
| タイトル | ||||||
| タイトル | 魚類の胚発生におけるオートファジーが関与する卵黄吸収機構 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Autophagy-Mediated Yolk Absorption during Fish Embryogenesis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Yolk absorption; proteolysis; autophagy; ubiquitin-proteasome pathway; zebrafi sh; microtubule-associated protein 1 light chain 3 （MAP1LC3） | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
今村, 伸太朗
× 今村, 伸太朗× 藪, 健史× 山下, 倫明 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Proteolytic degradation of yolk proteins is essential process for embryogenesis， homeostasis in fish. Since two different major pathways，i. e. ubiquitin-proteasome system and autophagy， have been well known in the animal cells， their roles for the yolk protein proteolysis during embryogenesis were characterized in the embryos of zebrafish and other fish species. Ubiquitin-proteasome system regulates ATP-dependent protein degradation specific to ubiquitinated proteins by proteasome， while autophagy regulates a process of bulky protein degradation via lysosomal/vacuolar system. The proteolytic processing of yolk protein in fish embryogenesis has been identified to be undertaken by lysosomal proteases. In order to characterize the molecular mechanisms on the proteolytic degradation of yolk protein， we examined roles of autophagy during embryogenesis. We established transgenic zebrafish lines， which are expressed a maker protein of autophagosome， microtubule-associated protein 1 light chain 3 （MAP1LC3） fused to green fluorescent protein （GFP）， to visualize autophagosome formed by autophagy. The transgenic embryos were very useful to observe autophagic cells in embryos， and we detected the induction of autophagosome during early embryogenesis. The treatment of embryos with protease inhibitors， such as E-64 （inhibitor of cathepsins B and L） and proteasome inhibitor， effectively repressed yolk protein degradation and embryogenesis， suggesting that both autophagy and proteasome pathways are essential for degradation of yolk proteins. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 水産総合研究センター研究報告 en : Bulletin of Fisheries Research Agency 巻 26, p. 29-34, ページ数 6, 発行日 2008-12 |
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| 出版者 | ||||||
| 出版者 | 水産総合研究センター | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1346-9894 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11589591 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | fra_k_26_29 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010770088 | |||||
| 関連名称 | 日本農学文献記事索引（AgriKnowledge） | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
