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Development and Evaluation of Real-Time Loop-Mediated Isothermal Amplification Methods for the Rapid Detection of Penaeid Viruses
https://fra.repo.nii.ac.jp/records/2010910
https://fra.repo.nii.ac.jp/records/2010910589e9fff-6d30-4720-8507-082d343b6985
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fra_k_35_39.pdf (1.2 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2024-10-02 | |||||||||
| タイトル | ||||||||||
| タイトル | Development and Evaluation of Real-Time Loop-Mediated Isothermal Amplification Methods for the Rapid Detection of Penaeid Viruses | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | RT-LAMP; WSSV; YHV; IHHNV; TSV; Penaeid shrimp; Japan | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 著者 |
米加田, 徹
× 米加田, 徹× SUDHAKARAN, Raja
× ITAMI, Toshiaki
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Shrimp viral diseases are a major impediment to commercial shrimp farming. The diseases affecting shrimp culture are white spot syndrome virus (WSSV), yellow head virus (YHV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), and Taura syndrome virus (TSV). Viral diseases are particularly difficult to control after the onset of infection. Therefore, prophylaxes to prevent or reduce the losses through vertical and horizontal transmission are most important. Various diagnostic methods have been developed to detect shrimp viral diseases, including bioassays, histopathology, polymerase chain reaction (PCR), and quantitative real-time PCR. Although the PCR-based methods are sensitive and highly specific, they require expensive equipment, costly reagents, and are time consuming. Therefore, a simple, quick and sensitive detection method is urgently needed to prevent the invasion of shrimp viral diseases into Japan from other countries. The loop-mediated isothermal amplification (LAMP) assay is a novel approach to amplify nucleic acid with high specificity, sensitivity, and rapidity under isothermal conditions, thereby obviating the need for a thermal cycler. Further, during the LAMP reaction, an insoluble byproduct, magnesium pyrophosphate, is produced in proportion to the large amounts of the target DNA amplified. Hence, real-time quantification can be achieved by measuring the turbidity of the magnesium pyrophosphate using an inexpensive photometer. This real-time LAMP method allows quantitative analysis of nucleic acid templates (real-time LAMP). In the present study, a comparatively less expensive quantitative real-time LAMP assay was successfully applied for detection of shrimp viral diseases and proven to have high sensitivity and specificity. | |||||||||
| 言語 | en | |||||||||
| 書誌情報 |
ja : 水産総合研究センター研究報告 en : Bulletin of Fisheries Research Agency 巻 35, p. 39-50, ページ数 12, 発行日 2012-01 |
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| 出版者 | ||||||||||
| 出版者 | 水産総合研究センター | |||||||||
| 言語 | ja | |||||||||
| ISSN | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 1346-9894 | |||||||||
| 書誌レコードID | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AA11589591 | |||||||||
| 情報源 | ||||||||||
| 識別子タイプ | Local | |||||||||
| 関連識別子 | fra_k_35_39 | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
