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マダイ筋原線維結合型セリンプロテアーゼの同定
https://fra.repo.nii.ac.jp/records/2012153
https://fra.repo.nii.ac.jp/records/2012153e37f5801-0fc5-43a6-a074-e1b22ce9562d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fishu_k_68_1.pdf (656.1 KB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2024-10-11 | |||||
| タイトル | ||||||
| タイトル | マダイ筋原線維結合型セリンプロテアーゼの同定 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Identification of Myofibril‒bound Serine Protease from Red Seabream（Pagrus major）Ordinary Muscle | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | red seabream; serine protease; modori; kamaboko | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
大久保, 誠
× 大久保, 誠× 谷口, 成紀× 近藤, 昌和× 前田, 俊道 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Myofibrillar proteins were prepared from red seabream (Pagrus major) ordinary muscle by washing four times to remove sarcoplasmic proteins. Myosin heavy chain (MHC) of the myofibrillar proteins was degraded at 55℃ without the addition of any protease, and the degree of MHC degradation in red seabream was obviously low in comparison with brushtooth lizardfish (Saurida sp.). Specific activity of the red seabream myofibrillar proteins was approximately one-thirtieth that of brushtooth lizardfish. Red seabream myofibril-bound serine protease (MBSP) was solubilized by heat treatment of the washed myofibrillar proteins at 55℃ for 10 min. Hydrolysis of synthetic fluorogenic substrate by both the myofibril-bound and solubilized MBSPs was inhibited by Pefabloc SC, a serine protease inhibitor. Both MBSPs rapidly hydrolyzed synthetic fluorogenic substrates containing arginine residues at the P1 position. These results indicate that red seabream MBSP is a trypsin-type serine protease, as reported in other fish species, such as white croaker (Pennahia argentata) and brushtooth lizardfish, utilized as ingredients of kamaboko. | |||||
| 言語 | en | |||||
| 書誌情報 |
ja : 水産大学校研究報告 en : Journal of National Fisheries University 巻 68, 号 1, p. 1-9, ページ数 9, 発行日 2019-11 |
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| 出版者 | ||||||
| 出版者 | 水産大学校 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | National Fisheries University | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0370-9361 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00124678 | |||||
| 情報源 | ||||||
| 識別子タイプ | Local | |||||
| 関連識別子 | fishu_k_68_1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://agriknowledge.affrc.go.jp/RN/2010932828 | |||||
| 言語 | en | |||||
| 関連名称 | agriknowledge | |||||
