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コイ鰾組織における血球観察のための固定および染色条件
https://doi.org/10.57348/0002014035
https://doi.org/10.57348/0002014035c26835a5-a94b-413f-ab69-10b01d837fdd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
fish-u_k_73-3_81.pdf (605.4 KB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
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| 公開日 | 2025-04-15 | |||||||||||
| タイトル | ||||||||||||
| タイトル | コイ鰾組織における血球観察のための固定および染色条件 | |||||||||||
| 言語 | ja | |||||||||||
| タイトル | ||||||||||||
| タイトル | Fixation and staining conditions of leukocytes observation in the koi carp swim bladder | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | carp; Cyprinus carpio; leukocyte; Bouin's solution; May-Grünwald·Giemsa | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.57348/0002014035 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | open access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
| 著者 |
安本, 信哉
× 安本, 信哉× 岡田, 理
× 近藤, 昌和 |
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Identification of leukocytes on tissue sections is important to elucidate the mechanism of swim bladder lesions. To determine the best fixative solution for a koi carp swim bladder, the swim bladders were fixed in 10% formalin, Bouin's, MFAA and Davidson's solution. The swim bladders fixed in MFAA or Davidson's solution were severely detached and twisted, whereas those fixed in 10% formalin and Bouin's solution kept their external shape. However, the majority of the 10% formalin-fixed specimens showed detachment of the tunica interna from the tunica externa under the light microscope. Therefore, Bouin’s solution was determined to be the most suitable fixing solution for the swim bladder. Imprints (head kidney-touched slides glass were fixed with Bouin's solution) and tissue sections of head kidney fixed in Bouin’s solution were stained with Mayer’s hematoxylin and eosin (HE) and May-Grünwald·Giemsa (MGG), and the best staining method for leukocytes identification was investigated. In the HE-stained specimens, identification of leukocytes by staining was difficult. On the other hand, MGG-stained specimens could be identified by staining. Fixation with Bouin's solution and MGG staining was determined to be the most suitable method for leukocytes identification in the swim bladder. | |||||||||||
| 言語 | en | |||||||||||
| 書誌情報 |
ja : 水産大学校研究報告 en : Journal of National Fisheries University 巻 73, 号 3, p. 81-85, ページ数 5, 発行日 2025-02 |
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| 出版者 | ||||||||||||
| 出版者 | 水産研究・教育機構 | |||||||||||
| 言語 | ja | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | Japan Fisheries Research and Education Agency | |||||||||||
| 言語 | en | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 0370-9361 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AN00124678 | |||||||||||
| 情報源 | ||||||||||||
| 識別子タイプ | Local | |||||||||||
| 関連識別子 | fish-u_k_73-3_81 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
